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SUMO1 Antibody (MA5-49789) in IHC (P)
Immunohistochemistry analysis with a SUMO1 monoclonal antibody (Product # MA5-49789) using a dilution of 1:92.5 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) w... View More
Product Details
MA5-49789
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5G3
Immunogen
A synthesized peptide derived from human SUMO1
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3092605
Target Information
SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. SUMO1 may also regulate a network of genes involved in palate development. Mutations in the gene can result in non-syndromic orofacial cleft 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CG4494-PA; GAP modifying protein 1; GAP-modifying protein 1; GMP1; LD07775p; OTTHUMP00000202403; OTTHUMP00000202404; OTTHUMP00000202405; OTTHUMP00000202406; OTTHUMP00000202409; OTTHUMP00000202410; Sentrin; Sentrin-1; Small ubiquitin-related modifier 1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; Smt3C; SUMO-1; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1
Gene Aliases: DAP1; GMP1; OFC10; OK/SW-cl.43; PIC1; SENP2; SMT3; SMT3C; SMT3H3; SUMO1; UBL1
UniProt ID: (Human) P63165
Entrez Gene ID: (Human) 7341
DNA repair
double-strand break repair via nonhomologous end joining
viral process
protein sumoylation
PML body organization
positive regulation of protein complex assembly
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of protein localization
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of action potential
negative regulation of transcription, DNA-templated
protein stabilization
palate development
regulation of interferon-gamma-mediated signaling pathway
global genome nucleotide-excision repair
regulation of cardiac muscle cell contraction
protein localization to nuclear pore
positive regulation of calcium-transporting ATPase activity
negative regulation of delayed rectifier potassium channel activity
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