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SWAP70 Antibody (PA5-78432) in ICC/IF
SWAP70 Polyclonal Antibody detects SWAP70 protein at cytoplasm by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SWAP70 protein stained by SWAP70 Polyclonal Antibody (Product # PA5-78432) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-78432
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human SWAP70. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736669
Product Specific Information
Positive Control: Jurkat, Raji, NCI-H929, 293T, A431, HeLa, HepG2
Predicted Reactivity: Rat (95%), Chicken (89%), Rhesus Monkey (99%), Bovine (93%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SWAP70 is a 585 amino acid regulatory protein belonging to the pleckstrin-like family with a PH domain essential for phosphatidylinositol 3, 4, 5-trisphosphate binding. SWAP70 acts as a link between the recognition of specific switch regions and causation of a DNA break and is involved in both nuclear events and in signaling B-cell activation. As a guanine nucleotide exchange factor (GEF) it transduces signals from tyrosine kinase receptors to RAC, independent of RAS. As a multiple functional signaling protein, and a component of SWAP enzyme complex, it mediates signaling of membrane ruffling by tyrosine kinase growth factor receptors, actin cytoskeleton and cell protrusion. It is widely expressed in most tissues, mature B-cells, including those associated with mucosa-associated tissue and bronchus-associated tissue, monocytes and macrophages.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ39540; KIAA0640 protein; SWAP complex protein, 70 kDa; SWAP-70; Switch-associated protein 70
Gene Aliases: 70kDa; AV235546; HSPC321; KIAA0640; SWAP-70; SWAP70
UniProt ID: (Human) Q9P061, (Mouse) Q6A028
Entrez Gene ID: (Human) 23075, (Mouse) 20947
positive regulation of cytosolic calcium ion concentration
negative regulation of actin filament depolymerization
positive regulation of actin filament bundle assembly
regulation of protein localization
negative regulation of cell-cell adhesion mediated by integrin
isotype switching
actin filament bundle assembly
positive regulation of mast cell chemotaxis
cell-cell adhesion
negative regulation of peptidyl-serine dephosphorylation
somatic cell DNA recombination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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