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Invitrogen
Sall1 Monoclonal Antibody (NRNSTNX), eFluor™ 450, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Sall1 Antibody (48-9729-82) in Flow
Demyelinated BALB/c mouse brain cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD11b Monoclonal Antibody, APC (Product # 17-0112-82) and 0.5 µg of Rat IgG2a kappa Isotype Control, eFluor 450 (Product # 48-4321-82) (left) or 0.5 µg of Sall1 Monoclonal Antibody, eFluor 450 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 506 (Product # 65-0866-18).
Product Details
48-9729-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
NRNSTNX
Immunogen
C-terminal fragment of the protein (aa-805-1322)
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848393
Product Specific Information
Description: This NRNSTNX monoclonal antibody recognizes mouse Sall1.
Applications Reported: This NRNSTNX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This NRNSTNX antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse brain cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Flow Protocols . This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Target Information
Sall1, which encodes a zinc finger protein, functions as a transcriptional repressor and interacts physically with histone deacetylase and other components of the chromatin remodeling NuRD complex. It is unknown whether the transcriptional repression is solely dependent on histone deacetylase activity. Gene expression profiling has identified Sall1 as a microglial signature gene. Microglia are the resident macrophages of the central nervous system (CNS). Sall1 is also expressed in abundance in the mesenchyme-derived structure from condensed mesenchyme, S-comma-shaped bodies, to renal tubules and podocytes. Sall1 has been identified as a key transcription factor in self-renewal renal progenitor cells. Sall1 is required to maintain the stemness of nephron progenitor cells by restraining their differentiation into renal vesicles. Defects in SALL1 are the cause of Townes-Brocks syndrome as well as bronchio-oto-renal syndrome. Heterozygous mutations of human SALL1 leading to Townes-Brocks syndrome features dysplastic ears, preaxial polydactyly, imperforate anus, and less commonly, kidney and heart anomalies (Kohlhase et al. 1998). Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: sal-3; sal-like protein 1; zinc finger protein Spalt-3
Gene Aliases: Msal-3
Entrez Gene ID: (Mouse) 58198
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
RNA polymerase II transcription coactivator activity
enhancer sequence-specific DNA binding
nucleic acid binding
DNA binding
chromatin binding
histone deacetylase activity
protein binding
beta-catenin binding
metal ion binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
branching involved in ureteric bud morphogenesis
kidney development
neural tube closure
ventricular septum development
mesenchymal to epithelial transition involved in metanephros morphogenesis
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
heart development
olfactory nerve development
olfactory bulb development
olfactory bulb interneuron differentiation
neural tube development
positive regulation of Wnt signaling pathway
inductive cell-cell signaling
forelimb morphogenesis
hindlimb morphogenesis
outer ear morphogenesis
embryonic digit morphogenesis
positive regulation of neuron differentiation
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract development
limb development
olfactory bulb mitral cell layer development
kidney epithelium development
ureteric bud invasion
mesenchymal stem cell maintenance involved in metanephric nephron morphogenesis
positive regulation of branching involved in ureteric bud morphogenesis
regulation of neural precursor cell proliferation
negative regulation of mesoderm development
negative regulation of ectoderm development
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