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Invitrogen
Salmonella Polyclonal Antibody
FIGURE: 1 / 3
Salmonella Antibody (PA1-7244) in ICC/IF
PloS one 2015 -
Figure 3 MDX promotes formation of enlarged Rab7 + Salmonella -containing vesicles. ( A ) Confocal micrographs of BMDM 90 minutes post-infection stained for Rab7 (green). Salmonella and nuclei stained with DAPI (blue). Scale bars: 10 uM ( B ) Quantitation of the number of Rab7 + vesicles per BMDM 90 minutes post-infection from confocal micrographs. Data represented as mean+-SEM. ( C ) Quantitation of the si... View More
Product Details
PA1-7244
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Published species
Bacteria,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mixture of S. enteriditis, S. typhimurium, and S. heidelburg.
Conjugate
Form
Liquid
Concentration
4-5 mg/mL
Purification
IgG fraction
Storage buffer
PBS, pH 7.2
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_561201
Product Specific Information
PA1-7244 detects Salmonella. This antibody is polyvalent for Salmonella "O & H" antigens. The antiserum is unabsorbed and does crossreact with related Enterobacteriaceae.
PA1-7244 has been successfully used in ELISA, and immunofluorescence procedures.
The PA1-7244 immunogen is a mixture of S. enteriditis, S. typhimurium, and S. heidelburg.
Recommended short term (less than 6 months) storage is 2-8ºC. For longer term storage, aliquot and freeze at -20ºC.
Target Information
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Salmonellae
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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