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Sclerostin Antibody (PA5-37943) in IHC (P)
Immunohistochemical analysis of Sclerostin in paraffin embedded human kidney using a Sclerostin polyclonal antibody (Product #PA5-37943) at a concentration of 4 µg/mL. Steamed antigen retrieval was performed with pH 9 buffered Tris and EDTA. Samples were then stained with horseradish peroxidase.
Product Details
PA5-37943
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-PRARSAKANQAELEN, from the C-terminus of SOST
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2554549
Product Specific Information
This antibody is predicted to react with mouse, rat, dog and cow based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
Sclerostin, a glycoprotein predominantly secreted by osteocytes, is a member of the Cerberus/DAN family of putative BMP antagonists that functions as an endogenous regulator of the canonical Wnt signaling pathway and an inhibitory regulator of bone homeostasis. Although expressed nearly exclusively by osteocytes, sclerostin can also be found at significant levels elsewhere, such as bone, bone marrow, cartilage, the kidney, and the liver, and has also been shown to be produced by hypertrophic chondrocytes and cementocytes. Like DKK family members DKK-1 and DKK-4, sclerostin plays an important regulatory role in the Wnt/beta-catenin signaling pathway by forming inhibitory complexes with LDL Receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/beta-catenin signaling system. LRP5 and LRP6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/beta-catenin signaling cascade. Sclerostin has also been shown to interact directly with LRP4 via its extracellular domain to facilitate inhibition of Wnt signaling, and can catabolically promote osteoclast activity by increasing osteocyte expression of RANKL. Sclerostin's critical involvement in the regulation of bone formation and resorption is emphasized by two bone dysplasia disorders, sclerosteosis and van Buchem disease (VBD), caused by rare autosomal recessive mutations that result in progressive bone overgrowth and hypermineralization due to markedly decreased sclerostin levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Sclerostin
Gene Aliases: CDD; DAND6; SOST; SOST1; UNQ2976/PRO7455/PRO7476; VBCH
UniProt ID: (Human) Q9BQB4
Entrez Gene ID: (Human) 50964
ossification
response to mechanical stimulus
Wnt signaling pathway
negative regulation of ossification
negative regulation of BMP signaling pathway
negative regulation of protein complex assembly
positive regulation of transcription, DNA-templated
cellular response to parathyroid hormone stimulus
negative regulation of canonical Wnt signaling pathway
negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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