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Sialosyl-Tn Antigen Antibody (MA1-90577) in WB
Western blot analysis of Sialosyl-Tn antigen was performed by loading 20 µg of human tissue lysates from the frontal cortex of 63-year-old (lane 1) and 85-year-old (lane 2) aged brains, per well onto a 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in PBST for 1 hour at room temperature. The membrane was probed with a Sialosyl-Tn antigen monoclonal antibody (Product # MA1-90577) at a dilution of 1:75 overnight at 4C, washed in PBST, and probed with an HRP-conjugated anti-mouse IgG second... View More
Product Details
MA1-90577
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Sheep
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
STn 219
Immunogen
Ovine submaxillary mucin (OSM)
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2264594
Product Specific Information
Recommended positive controls: Human gastrointestinal tumor, prostate, ovary ca..
This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane and luminal staining patterns. It is negative with majority of adult tissue sections.
Target Information
Sialosyl Tn antigen, also known as CD175s, is expressed in the majority of colon, gastric, breast, and lung carcinoma. The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD175s
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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