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Invitrogen
Siglec-G Monoclonal Antibody (SH2.1), APC, eBioscience™
FIGURE: 1 / 1
Siglec-G Antibody (17-5833-82) in Flow
Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or 0.06 µg of Anti-Mouse Siglec G APC (right). Cells in the lymphocyte gate were used for analysis.
Product Details
17-5833-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SH2.1
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573230
Product Specific Information
Description: The SH2.1 monoclonal antibody reacts with mouse siglec-G, which is also known as sialic acid-binding immunoglobulin-like lectin-G. Siglec-G is an inhibitory receptor that is expressed in a B cell-specific manner with B1 B cells expressing the highest levels of siglec-G. When overexpressed, siglec-G inhibits B cell receptor-mediated calcium signaling. Siglec-G-deficient mice show a massive expansion of the B1a cell population as well as higher titers of natural IgM antibodies. Siglec-G-deficient B1a cells show a lower level of spontaneous apoptosis and a prolonged life span, which may result from higher expression levels of the transcription factor NFATc1. Siglec-G-dependent negative regulation exists in B1 cells, which may explain the naturally muted signaling response of B1 cells.
Applications Reported: This SH2.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SH2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Siglec-G is an inhibitory receptor that is expressed in a B cell-specific manner with B1 B cells expressing the highest levels of siglec-G. When overexpressed, siglec-G inhibits B cell receptor-mediated calcium signaling. Siglec-G-deficient mice show a massive expansion of the B1a cell population as well as higher titers of natural IgM antibodies. Siglec-G-deficient B1a cells show a lower level of spontaneous apoptosis and a prolonged life span, which may result from higher expression levels of the transcription factor NFATc1. Siglec-G-dependent negative regulation exists in B1 cells, which may explain the naturally muted signaling response of B1 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: sialic acid binding Ig-like lectin 10; Sialic acid-binding Ig-like lectin 10; Sialic acid-binding Ig-like lectin G; Siglec; Siglec 10; Siglec-10; Siglec-G
Gene Aliases: 9830164H23; A630096C01Rik; mSiglec-G; Siglec-G; Siglec10; Siglecg
UniProt ID: (Mouse) Q80ZE3
Entrez Gene ID: (Mouse) 243958
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