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Sin3A Antibody (A300-725A) in WB
Detection of human Sin3A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Sin3A antibody A300-725A (lot A300-725A-2) used for IP at 6 µg per reaction. Sin3A was also immunoprecipitated by a previous lot of this antibody (lot A300-725A-1) and rabbit anti-Sin3A antibodies A300-724A and A300-726A. For blotting immunoprecipitated Sin3A, A300-725A was used at 1 µg/ml.... View More
Product Details
A300-725A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 375 and 425 of Human SIN3 homolog A.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Histone deacetylase complex subunit Sin3a; Mammalian Sin3A; Paired amphipathic helix protein Sin3a; SIN; SIN3 homolog A, transcription regulator; SIN3 transcription regulator homolog A; SIN3A; transcriptional co-repressor Sin3A; Transcriptional corepressor Sin3a; transcriptional regulator, SIN3A
Gene Aliases: SIN3A
UniProt ID: (Human) Q96ST3
Entrez Gene ID: (Human) 25942
transcription regulatory region sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
RNA polymerase II repressing transcription factor binding
RNA polymerase II transcription corepressor activity
chromatin binding
transcription factor activity, sequence-specific DNA binding
RNA binding
histone deacetylase activity
protein binding
protein complex binding
protein deacetylase activity
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
activation of innate immune response
positive regulation of defense response to virus by host
hematopoietic progenitor cell differentiation
DNA replication
transcription, DNA-templated
protein deacetylation
aging
regulation of hormone levels
positive regulation of G2/M transition of mitotic cell cycle
histone deacetylation
positive regulation of chromatin silencing
cellular protein localization
negative regulation of circadian rhythm
negative regulation of apoptotic process
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
cellular lipid metabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
response to methylglyoxal
cellular response to glucose stimulus
negative regulation of protein localization to nucleus
negative regulation of histone H3-K27 acetylation
cellular response to dopamine
negative regulation of transcription regulatory region DNA binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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