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Skeletal Muscle Actin Antibody (PA5-114172) in WB
Western Blot of Mouse using a Polyclonal Skeletal Muscle Actin Antibody (Product # PA5-114172). Lane 1: 20 µg mouse GL261 cell lysate. Lane 2: 20 µg mouse astrocyte cell lysate. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:500.
Product Details
PA5-114172
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Immunogen sequence: GQKDSYVGDE AQSKRGILTL KYPIEHGIIT NWDDMEKIWH HTFYNELRVA
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2884687
Product Specific Information
Immunogen sequence: GQKDSYVGDE AQSKRGILTL KYPIEHGIIT NWDDMEKIWH HTFYNELRVA
For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Predicted homology: Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Rabbit: 100%; Rat: 100%
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin, alpha skeletal muscle; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha-actin-1; Alpha-actin-2; alpha-cardiac actin; Cell growth-inhibiting gene 46 protein; nemaline myopathy type 3
Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACTA; Acta-2; ACTA1; ACTA2; Acts; ACTSA; Actsk-1; ACTVS; alphaSMA; ASMA; CFTD; CFTD1; CFTDM; GIG46; MPFD; MYMY5; NEM1; NEM2; NEM3; SHPM; SMalphaA
UniProt ID: (Human) P68133, (Human) P62736, (Mouse) P68134, (Mouse) P62737
Entrez Gene ID: (Human) 58, (Human) 59, (Mouse) 11459, (Mouse) 11475
muscle contraction
response to mechanical stimulus
response to extracellular stimulus
response to lithium ion
positive regulation of gene expression
cell growth
muscle filament sliding
skeletal muscle thin filament assembly
skeletal muscle fiber adaptation
response to steroid hormone
skeletal muscle fiber development
mesenchyme migration
regulation of blood pressure
response to virus
vascular smooth muscle contraction
glomerular mesangial cell development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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