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Slc30A8 Antibody (PA5-103252) in ICC/IF
Immunofluorescent analysis of Slc30A8 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Slc30A8 polyclonal antibody (Product # PA5-103252) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-103252
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SLC30A8(Accession Q8IWU4), corresponding to amino acid residues I179-I229.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852615
Product Specific Information
Antibody detects endogenous levels of total SLC30A8.
Target Information
Slc30A8 is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles and is expressed at a high level in the pancreas, particularly in islets of Langerhans, and co-localizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of Slc30A8 have been identified as major genetic risk factors for the development of Type 2 diabetes, but no genetic association has been found with Type 1 diabetes. Slc30A8 expression in b-cells was found to be influenced by cytokine expression, particularly IFN-gamma and IL-1 beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Proton-coupled zinc antiporter SLC30A8; solute carrier family 30 (zinc transporter), member 8; Solute carrier family 30 member 8; Zinc transporter 8; zinc transporter ZnT-8; ZnT-8
Gene Aliases: SLC30A8; ZnT-8; ZNT8
UniProt ID: (Human) Q8IWU4
Entrez Gene ID: (Human) 169026
zinc II ion transport
cellular zinc ion homeostasis
response to glucose
response to zinc ion
insulin secretion
positive regulation of insulin secretion
sequestering of zinc ion
response to interferon-gamma
glucose homeostasis
regulation of vesicle-mediated transport
regulation of sequestering of zinc ion
response to interleukin-1
zinc II ion transmembrane transport
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