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Smac/Diablo Antibody (100-401-244) in WB
Western blot using anti-Smac detects a 26 kDa band when 1 µg of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 µg of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 µg each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 µM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution... View More
Product Details
100-401-244
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This whole rabbit serum was prepared by repeated immunizations with recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239).
Conjugate
Form
Liquid
Concentration
85 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antiserum is directed against human Smac/DIABLO and is useful in determining its presence in various assays.
Target Information
DIABLO (smac), a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms. Smac/DIABLO has also been found to play a key role in regulating the sensitization of cancer cells to apoptosis (both immune and drug-induced).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Diablo homolog, mitochondrial; Diablo IAP-binding mitochondrial protein; diablo, IAP-binding mitochondrial protein; diablo-like protein; DIABLO-S; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; hCG_1782202; second mitochondria-derived activator of caspase; Second mitochondria-derived activator of caspases; SMAC; SMAC3
Gene Aliases: DFNA64; DIABLO; SMAC
UniProt ID: (Human) Q9NR28
Entrez Gene ID: (Human) 56616
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to oxidative stress
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
positive regulation of apoptotic process
neuron apoptotic process
intrinsic apoptotic signaling pathway
Performance Guarantee
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