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Smad1 Antibody (600-401-MN6) in ICC/IF
Immunofluorescence of Rabbit Anti-Smad1 Antibody. Cells: C2C12 cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-Smad1 at 10 µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Rabbit IgG DyLight™488 (p/n 611-741-127) at 5 µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-Smad1 + DyLight™488. (C) Merge A+B. (D) Secondary Only. Expected localization: Nucleus, Plasma membrane, and Cytosol.
Product Details
600-401-MN6
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Smad1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Smad1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Smad1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 83% homology to human and rat.
Target Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dwarfin-A; Dwf-A; DWFC; hSMAD1; MAD homolog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD5
Gene Aliases: AI528653; Madh1; Madr1; Mlp1; MusMLP; Smad1
UniProt ID: (Mouse) P70340
Entrez Gene ID: (Mouse) 17125
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
identical protein binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
DNA-binding transcription factor
gene-specific transcriptional regulator
MAPK cascade
mesodermal cell fate commitment
osteoblast fate commitment
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein phosphorylation
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
gamete generation
negative regulation of cell proliferation
positive regulation of gene expression
BMP signaling pathway
midbrain development
hindbrain development
response to drug
homeostatic process
positive regulation of cell differentiation
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
cardiac muscle cell proliferation
bone development
positive regulation of cartilage development
cellular response to organic cyclic compound
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
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