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Sox17 Antibody (CF500044) in ICC/IF
Figure from citation: Immunofluorescence of SOX17 protein level by using anti-SOX17 antibody in human KhES-3 cells. View Citation
Product Details
CF500044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI3B10
Immunogen
Human recombinant protein fragment corresponding to amino acids 177-414 of human SOX17 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
SOX17 (transcription factor SOX-17) belongs to the SRY-related HMB box family of transcription factors. It acts as a transcription regulator that binds target promoter DNA and bends the DNA. It binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. SOX17 modulates transcriptional regulation via WNT3A and inhibits Wnt signaling. It promotes degradation of activated CTNNB1. SOX17 plays an essential role in the regulation of embryonic development and is required for normal looping of the embryonic hear tube and for normal development of the definitive gut endoderm. Mutations in the gene can result in vesicoureteral reflux.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SRY (sex determining region Y)-box 17; SRY box 17; SRY-related HMG-box transcription factor SOX17; Transcription factor SOX-17
Gene Aliases: SOX17; VUR3
UniProt ID: (Human) Q9H6I2
Entrez Gene ID: (Human) 64321
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
protein binding
beta-catenin binding
transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
HMG box transcription factor
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
vasculogenesis
endoderm formation
inner cell mass cellular morphogenesis
heart looping
cardiogenic plate morphogenesis
embryonic heart tube morphogenesis
outflow tract morphogenesis
negative regulation of Wnt signaling pathway involved in heart development
regulation of transcription, DNA-templated
spermatogenesis
endodermal cell fate determination
rostrocaudal neural tube patterning
signal transduction involved in regulation of gene expression
negative regulation of cell growth
protein destabilization
embryonic heart tube development
cell migration involved in gastrulation
negative regulation of mesodermal cell fate specification
mRNA transcription from RNA polymerase II promoter
positive regulation of cell differentiation
positive regulation of protein catabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of embryonic development
embryonic foregut morphogenesis
positive regulation of skeletal muscle tissue development
stem cell fate specification
protein stabilization
canonical Wnt signaling pathway
endocardium formation
regulation of transcription from RNA polymerase II promoter involved in definitive endodermal cell fate specification
cardiac cell fate determination
heart formation
endocardial cell differentiation
common bile duct development
gall bladder development
endodermal digestive tract morphogenesis
renal system development
regulation of stem cell proliferation
negative regulation of canonical Wnt signaling pathway
regulation of stem cell division
regulation of cardiac cell fate specification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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