Invitrogen

Survivin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

9 Published Figures

11 References

View all (86) Survivin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Survivin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

Survivin Antibody (PA1-16836) in ICC/IF

Immunocytochemistry analysis of Survivin in HeLa cells. Samples were incubated in Survivin polyclonal antibody (Product # PA1-16836) using a dilution of 1:10 followed by Alexa Fluor 488-conjugated Goat to rabbit IgG secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Survivin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Survivin Antibody in Immunohistochemistry (IHC)

Survivin Antibody in Western Blot, Immunoprecipitation (WB, IP)

Survivin Antibody in Western Blot, ChIP Assay (WB, ChIP)

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Product Details

PA1-16836

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:50-1:250

Flow Cytometry (Flow)

Assay-Dependent

ELISA (ELISA)

Assay-Dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

1:10-1:500

View 1 publication 1 publication

ChIP assay (ChIP)

1:10-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Cat, Guinea pig, Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human survivin, full-length.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568741

Product Specific Information

For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin.

Suggested positive control: Hela whole cell extract, antigen standard for BIRC5 (transient overexpression lysate).

Target Information

Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin’s gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Suppression of phosphodiesterase IV enzyme by roflumilast ameliorates cognitive dysfunction in aged rats after sevoflurane anaesthesia via PKA-CREB and MEK/ERK pathways.

The European journal of neuroscience

Liu P,Wang J,Peng S,Zhang D,Zhuang L,Liu C,Zhang Y,Shi X

Published figure using Survivin polyclonal antibody (Product # PA1-16836) in Immunohistochemistry

Mon Aug 01 00:00:00 EDT 2022

Suppression of phosphodiesterase IV enzyme by roflumilast ameliorates cognitive dysfunction in aged rats after sevoflurane anaesthesia via PKA-CREB and MEK/ERK pathways.

The European journal of neuroscience

Liu P,Wang J,Peng S,Zhang D,Zhuang L,Liu C,Zhang Y,Shi X

Published figure using Survivin polyclonal antibody (Product # PA1-16836) in Immunohistochemistry

Mon Aug 01 00:00:00 EDT 2022

In vivo analysis of onset and progression of retinal degeneration in the Nr2e3^rd7/rd7 mouse model of enhanced S-cone sensitivity syndrome.

Scientific reports

Venturini G,Kokona D,Steiner BL,Bulla EG,Jovanovic J,Zinkernagel MS,Escher P

PA1-16836 was used in Immunohistochemistry and Western Blotting to characterize in detail C57BL/6J Nr2e3rd7/rd7 mice in vivo.

Fri Sep 24 00:00:00 EDT 2021

In vivo analysis of onset and progression of retinal degeneration in the Nr2e3^rd7/rd7 mouse model of enhanced S-cone sensitivity syndrome.

Scientific reports

Venturini G,Kokona D,Steiner BL,Bulla EG,Jovanovic J,Zinkernagel MS,Escher P

PA1-16836 was used in Immunohistochemistry and Western Blotting to characterize in detail C57BL/6J Nr2e3rd7/rd7 mice in vivo.

Fri Sep 24 00:00:00 EDT 2021

In vivo analysis of onset and progression of retinal degeneration in the Nr2e3^rd7/rd7 mouse model of enhanced S-cone sensitivity syndrome.

Scientific reports

Venturini G,Kokona D,Steiner BL,Bulla EG,Jovanovic J,Zinkernagel MS,Escher P

PA1-16836 was used in Immunohistochemistry and Western Blotting to characterize in detail C57BL/6J Nr2e3rd7/rd7 mice in vivo.

Fri Sep 24 00:00:00 EDT 2021

BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.

Autophagy

Lin TY,Chan HH,Chen SH,Sarvagalla S,Chen PS,Coumar MS,Cheng SM,Chang YC,Lin CH,Leung E,Cheung CHA

Published figure using Survivin polyclonal antibody (Product # PA1-16836) in Immunoprecipitation

Wed Jul 01 00:00:00 EDT 2020

BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.

Autophagy

Lin TY,Chan HH,Chen SH,Sarvagalla S,Chen PS,Coumar MS,Cheng SM,Chang YC,Lin CH,Leung E,Cheung CHA

Published figure using Survivin polyclonal antibody (Product # PA1-16836) in Immunoprecipitation

Wed Jul 01 00:00:00 EDT 2020

BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.

Autophagy

Lin TY,Chan HH,Chen SH,Sarvagalla S,Chen PS,Coumar MS,Cheng SM,Chang YC,Lin CH,Leung E,Cheung CHA

Published figure using Survivin polyclonal antibody (Product # PA1-16836) in Immunoprecipitation

Wed Jul 01 00:00:00 EDT 2020

Cancer testis antigen expression across T-cell lymphoma subtypes.

Hematological oncology

Ma H,Soderquist CR,Marchi E,Scotto L,Bhagat G,O'Connor OA

PA1-16836 was used in Immunohistochemistry to study cancer testis antigen expression across T-cell lymphoma subtypes.

Tue Dec 01 00:00:00 EST 2020

Cancer testis antigen expression across T-cell lymphoma subtypes.

Hematological oncology

Ma H,Soderquist CR,Marchi E,Scotto L,Bhagat G,O'Connor OA

PA1-16836 was used in Immunohistochemistry to study cancer testis antigen expression across T-cell lymphoma subtypes.

Tue Dec 01 00:00:00 EST 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

PA1-16836 was used in Western Blotting to show that pFUS may be a strategy for enhancing the therapeutic efficacy of MSC-derived EV treatment for the treatment of AKI.

Mon Sep 14 00:00:00 EDT 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

PA1-16836 was used in Western Blotting to show that pFUS may be a strategy for enhancing the therapeutic efficacy of MSC-derived EV treatment for the treatment of AKI.

Mon Sep 14 00:00:00 EDT 2020

The expression of survivin in irreversible pulmonary arterial hypertension rats and its value in evaluating the reversibility of pulmonary arterial hypertension secondary to congenital heart disease.

Pulmonary circulation

Li G,Zhang H,Zhao L,Zhang Y,Yan D,Liu Y,Su J,Fan X

PA1-16836 was used in an ELISA assay to the increased survivin level is a feature of irreversible PAH and the serum survivin represents a candidate biomarker reflecting the operability of CHD-PAH patients.

Wed Sep 30 00:00:00 EDT 2020

Hepatitis B virus X protein accelerates hepatocarcinogenesis with partner survivin through modulating miR-520b and HBXIP.

Molecular cancer

Zhang W,Lu Z,Kong G,Gao Y,Wang T,Wang Q,Cai N,Wang H,Liu F,Ye L,Zhang X

PA1-16836 was used in western blot to investigate hepatocarcinogenesis mediated by hepatitis B virus X protein

Wed May 28 00:00:00 EDT 2014

Hepatitis B virus X protein accelerates hepatocarcinogenesis with partner survivin through modulating miR-520b and HBXIP.

Molecular cancer

Zhang W,Lu Z,Kong G,Gao Y,Wang T,Wang Q,Cai N,Wang H,Liu F,Ye L,Zhang X

PA1-16836 was used in western blot to investigate hepatocarcinogenesis mediated by hepatitis B virus X protein

Wed May 28 00:00:00 EDT 2014

NR4A1 enhances neural survival following oxygen and glucose deprivation: an in vitro study.

Journal of the neurological sciences

Xiao G,Sun T,Songming C,Cao Y

PA1-16836 was used in western blot to study the ability of NR4A1 to protect against neural damage induced by oxygen and glucose deprivation

Mon Jul 15 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat containing 5; baculoviral IAP repeat-containing 5; Baculoviral IAP repeat-containing protein 5; BIRC 5; EPR 1; inhibitor of apoptosis; survivin; survivin variant 3 alpha; SVV; TIAP

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Gene Aliases: AAC-11; AP14; API4; BIRC5; EPR-1; IAP4; survivin40; TIAP

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UniProt ID: (Dog) Q8I009, (Cat) Q6I6F4, (Human) O15392, (Rat) Q9JHY7, (Guinea pig) E3SCZ8, (Mouse) O70201

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Entrez Gene ID: (Dog) 442936, (Cat) 493835, (Human) 332, (Rat) 64041, (Guinea pig) 100527953, (Mouse) 11799, (Chinese hamster) 100689366

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Function(s)

microtubule binding zinc ion binding tubulin binding protein homodimerization activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process ubiquitin-protein transferase activity cysteine-type endopeptidase inhibitor activity protein binding Ran GTPase binding enzyme binding identical protein binding metal ion binding chaperone binding molecular_function protein heterodimerization activity cofactor binding peptidase inhibitor activity deacetylase

Process(es)

G2/M transition of mitotic cell cycle mitotic cytokinesis protein phosphorylation apoptotic process chromosome segregation mitotic spindle assembly checkpoint protein complex localization positive regulation of exit from mitosis negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of transcription, DNA-templated positive regulation of mitotic cell cycle cell division establishment of chromosome localization transcription, DNA-templated sister chromatid cohesion positive regulation of cell proliferation regulation of signal transduction protein ubiquitination protein sumoylation regulation of apoptotic process mitotic spindle assembly inhibition of cysteine-type endopeptidase activity involved in apoptotic process microtubule cytoskeleton organization regulation of mitotic cell cycle cellular response to stem cell factor stimulus response to drug response to peptide hormone negative regulation of neuron apoptotic process positive regulation of cell cycle response to glucocorticoid regulation of insulin secretion involved in cellular response to glucose stimulus regulation of type B pancreatic cell proliferation cellular response to ethanol cytokinesis regulation of transcription, DNA-templated cell cycle mitotic nuclear division embryo development negative regulation of peptidase activity spindle checkpoint

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Survivin Polyclonal Antibody

Survivin Antibody (PA1-16836) in ICC/IF

Product Details

PA1-16836

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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