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Invitrogen
Survivin Recombinant Rabbit Monoclonal Antibody (9H18L32)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 12
Survivin Antibody (700387) in ICC/IF
Immunofluorescence analysis of Survivin was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Survivin Recombinant Rabbit Monoclonal Antibody (9H18L32) (Product # 700387) at 5 µg/mL in 0.1% BSA and incubated for overnight at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluo... View More
Product Details
700387
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
9H18L32
Immunogen
A recombinant protein corresponding to amino acids 1-142.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532319
Product Specific Information
This antibody is predicted to react with bovine and hamster based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin’s gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat containing 5; baculoviral IAP repeat-containing 5 (survivin); Baculoviral IAP repeat-containing protein 5; BIRC 5; EPR 1; survivin; survivin variant 3 alpha; SVV; TIAP
Gene Aliases: AAC-11; AP14; API4; BIRC5; EPR-1; IAP4; survivin40; TIAP
UniProt ID: (Bovine) Q6J1J1, (Human) O15392, (Rat) Q9JHY7, (Mouse) O70201
Entrez Gene ID: (Bovine) 414925, (Human) 332, (Rat) 64041, (Mouse) 11799, (Chinese hamster) 100689366
microtubule binding
zinc ion binding
tubulin binding
protein homodimerization activity
cysteine-type endopeptidase inhibitor activity involved in apoptotic process
ubiquitin-protein transferase activity
cysteine-type endopeptidase inhibitor activity
protein binding
Ran GTPase binding
enzyme binding
identical protein binding
metal ion binding
chaperone binding
molecular_function
protein heterodimerization activity
cofactor binding
peptidase inhibitor activity
G2/M transition of mitotic cell cycle
microtubule cytoskeleton organization
transcription, DNA-templated
protein phosphorylation
chromosome segregation
regulation of mitotic cell cycle
regulation of signal transduction
protein ubiquitination
protein complex localization
positive regulation of exit from mitosis
cellular response to stem cell factor stimulus
response to drug
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to peptide hormone
negative regulation of neuron apoptotic process
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of mitotic cell cycle
cell division
establishment of chromosome localization
response to glucocorticoid
regulation of insulin secretion involved in cellular response to glucose stimulus
regulation of type B pancreatic cell proliferation
cellular response to ethanol
mitotic spindle assembly
inhibition of cysteine-type endopeptidase activity involved in apoptotic process
mitotic cytokinesis
apoptotic process
mitotic spindle assembly checkpoint
sister chromatid cohesion
positive regulation of cell proliferation
protein sumoylation
regulation of apoptotic process
cytokinesis
regulation of transcription, DNA-templated
cell cycle
mitotic nuclear division
embryo development
negative regulation of peptidase activity
spindle checkpoint
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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