Invitrogen

Syk Monoclonal Antibody (4D10.1), eBioscience™

1 Published Figure
2 References
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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

14-6696-82

Applications
Tested Dilution
Publications

Western Blot (WB)

5 µg/mL
-

Flow Cytometry (Flow)

Assay-Dependent
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4D10.1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718976

Product Specific Information

Description: This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is expressed most highly in B cells, with lower expression in immature thymocytes, mast cells, and platelets. Syk can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells. This protein is a major component of signaling cascades downstream of the B and T cell antigen receptors and plays an essential role in lymphocyte development. Upon recruitment to immunoreceptor tyrosine-based activation motifs (ITAMs) on the antigen receptors, Syk is activated by phosphorylation on multiple tyrosines. Once activated, Syk phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Finally, abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers.

Applications Reported: This 4D10.1 antibody has been reported for use in flow cytometric analysis and western blotting.

Applications Tested: This 4D10.1 antibody has been tested by immunoblotting of reduced Ramos cell lysate. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

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Gene Aliases: p72-Syk; SYK

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UniProt ID: (Human) P43405

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Entrez Gene ID: (Human) 6850

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity integrin binding protein binding ATP binding protein kinase binding
Process(es)
angiogenesis lymph vessel development positive regulation of receptor internalization stimulatory C-type lectin receptor signaling pathway adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response leukocyte activation involved in immune response serotonin secretion by platelet protein phosphorylation inflammatory response leukocyte cell-cell adhesion transmembrane receptor protein tyrosine kinase signaling pathway integrin-mediated signaling pathway activation of JUN kinase activity cell proliferation organ morphogenesis regulation of platelet activation regulation of tumor necrosis factor-mediated signaling pathway viral process peptidyl-serine phosphorylation leukotriene biosynthetic process platelet activation neutrophil chemotaxis receptor internalization positive regulation of type I interferon production regulation of superoxide anion generation positive regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis defense response to bacterium transcription factor import into nucleus positive regulation of mast cell degranulation regulation of neutrophil degranulation beta selection innate immune response positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of bone resorption positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation blood vessel morphogenesis positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation regulation of phagocytosis positive regulation of calcium-mediated signaling B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of ERK1 and ERK2 cascade cellular response to molecule of fungal origin cellular response to low-density lipoprotein particle stimulus regulation of arachidonic acid secretion regulation of platelet aggregation
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