Invitrogen

Syk Monoclonal Antibody (SYK-01)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4 Published Figures

5 References

View all (53) Syk antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Syk Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

Syk Antibody (MA1-19332) in ICC/IF

Immunofluorescence of SYK was performed using THP-1 wild-type and SYK KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the SYK monoclonal antibody (P... View More

Syk Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

Syk Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Higher expression of Syk was observed specifically in B-lymphocytes (Ramos) in comparison to other hematopoietic cell lines (THP-1, Jurkat) using Anti-Syk Monoclonal Antibody (Product # MA1-19332) in western blot. {RE}

Product Details

MA1-19332

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SYK-01

Immunogen

Recombinant fragment (aa 5-360) of human Syk.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2197214

Product Specific Information

This antibody reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).

Target Information

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuronal C-Reactive Protein/FcγRI Positive Feedback Proinflammatory Signaling Contributes to Nerve Injury Induced Neuropathic Pain.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Liu F,Zhang L,Su S,Fang Y,Yin XS,Cui H,Sun J,Xie Y,Ma C

MA1-19332 was used in Immunohistochemistry-immunofluorescence to provide novel insights into the neuroimmune responses after peripheral nerve injury and suggest potential therapeutic targets for neuropathic pain.

Sat Apr 01 00:00:00 EDT 2023

Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway.

Aging cell

Jung ES,Suh K,Han J,Kim H,Kang HS,Choi WS,Mook-Jung I

MA1-19332 was used in Western Blot to suggest that repurposing of FA for inhibiting microglial activation of the NLRP3 inflammasome is a potential treatment for AD.

Sun May 01 00:00:00 EDT 2022

Amyloid-β activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway.

Aging cell

Jung ES,Suh K,Han J,Kim H,Kang HS,Choi WS,Mook-Jung I

MA1-19332 was used in Western Blot to suggest that repurposing of FA for inhibiting microglial activation of the NLRP3 inflammasome is a potential treatment for AD.

Sun May 01 00:00:00 EDT 2022

An Allosteric Shift in CD11c Affinity Activates a Proatherogenic State in Arrested Intermediate Monocytes.

Journal of immunology (Baltimore, Md. : 1950)

Hernandez AA,Foster GA,Soderberg SR,Fernandez A,Reynolds MB,Orser MK,Bailey KA,Rogers JH,Singh GD,Wu H,Passerini AG,Simon SI

MA1-19332 was used in Western Blotting to conclude that CD11c functions as a mechanoregulator that activates an inflammatory state preferentially in a majority of iMo from cardiac patients but not healthy patients.

Sun Nov 15 00:00:00 EST 2020

CARD9 negatively regulates NLRP3-induced IL-1β production on Salmonella infection of macrophages.

Nature communications

Pereira M,Tourlomousis P,Wright J,P Monie T,Bryant CE

MA1-19332 was used in immunoprecipitation and western blot to examine the contribution of the CARD9 domain of interleukin-1beta in Salmonella enterica serovar Typhimurium infection

Tue Sep 27 00:00:00 EDT 2016

CARD9 negatively regulates NLRP3-induced IL-1β production on Salmonella infection of macrophages.

Nature communications

Pereira M,Tourlomousis P,Wright J,P Monie T,Bryant CE

MA1-19332 was used in immunoprecipitation and western blot to examine the contribution of the CARD9 domain of interleukin-1beta in Salmonella enterica serovar Typhimurium infection

Tue Sep 27 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; p72-Syk; p72Syk; SP1; Spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; Tyrosine-protein kinase SYK

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Gene Aliases: p72-Syk; p72syk; ptk72; SYK; Sykb

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UniProt ID: (Human) P43405, (Rat) Q64725, (Mouse) P48025

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Entrez Gene ID: (Human) 6850, (Rat) 25155, (Mouse) 20963

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity integrin binding protein binding ATP binding protein kinase binding receptor binding protein domain specific binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups kinase modulator

Process(es)

Performance Guarantee

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Syk Monoclonal Antibody (SYK-01)

Syk Antibody (MA1-19332) in ICC/IF

Product Details

MA1-19332

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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