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Invitrogen
Synaptophysin Monoclonal Antibody (EP10), eFluor™ 660, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Synaptophysin Antibody (50-6525-82) in ICC/IF
Immunofluorescence analysis of Synaptophysin was performed using SH-SY5Y cells differentiated into neurons. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Synaptophysin Monoclonal Antibody (EP10), eFluor 660, eBioscience™ (Product # 50-6525-82) at 5 µg/mL i... View More
Product Details
50-6525-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
EP10
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574271
Product Specific Information
Description: This EP10 monoclonal antibody reacts with human Synaptophysin, which is also known as Major Synaptic Vesicle Protein p38. This 38-kDa integral membrane protein is expressed on neuronal presynaptic vesicles in the brain, spinal cord, retina, neuromuscular junctions, and adrenal medulla. Synaptophysin is also expressed in neuroendocrine tumors and neuroblastomas. Synaptophysin has been reported to play a role in synaptic vesicle trafficking.
The EP10 antibody recognizes human and hamster synaptophysin but does not recognize mouse and rat synaptophysin.
Applications Reported: This EP10 antibody has been reported for use in immunohistochemical staining, and immunocytochemistry.
Applications Tested: This EP10 antibody has been tested by immunohistochemistry on FFPE human tissue using low pH antigen retrieval at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Major synaptic vesicle protein p38; Synaptophysin
Gene Aliases: I79_012252; MRX96; MRXSYP; SYP
UniProt ID: (Human) P08247
Entrez Gene ID: (Human) 6855, (Chinese hamster) 100758282
endocytosis
synaptic vesicle maturation
regulation of neuronal synaptic plasticity
regulation of long-term neuronal synaptic plasticity
regulation of short-term neuronal synaptic plasticity
synaptic vesicle membrane organization
cellular response to organic substance
regulation of opioid receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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