Invitrogen

Synaptophysin Monoclonal Antibody (SY38)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

4 Published Figures

8 References

View all (95) Synaptophysin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Synaptophysin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

FIGURE: 1 / 9

Synaptophysin Antibody (MA1-213) in IHC (PFA)

Immunohistochemistry was performed on human cerebellum and human breast tissue. Tissue was deparaffinized with xylene, followed by rehydration in sequential washes of 100% ethanol, 95% ethanol, 80% ethanol, and water. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and heated for 20 min. Following antigen retrieval, tissues were blocked in a 10% goat serum (Prod... View More

Synaptophysin Antibody in Western Blot (WB)

Synaptophysin Antibody in Flow Cytometry (Flow)

Synaptophysin Antibody in Immunohistochemistry (IHC)

Synaptophysin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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Product Details

MA1-213

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:1,500

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

1:200-1:500

Immunoprecipitation (IP)

- -

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SY38

Immunogen

Synaptophysin from presynaptic vesicles from bovine brain

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2723681

Product Specific Information

MA1-213 has been successfully used in western blot, imunofluorescence, immunohistochemistry, flow cytometry, immunoprecipitation and ELISA.

MA1-213 detects Synaptophysin in human, bovine, mouse and rat samples.

Target Information

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Postoperative risk of IDH-mutant glioma-associated seizures and their potential management with IDH-mutant inhibitors.

The Journal of clinical investigation

Drumm MR,Wang W,Sears TK,Bell-Burdett K,Javier R,Cotton KY,Webb B,Byrne K,Unruh D,Thirunavu V,Walshon J,Steffens A,McCortney K,Lukas RV,Phillips JJ,Mohamed E,Finan JD,Santana-Santos L,Heimberger AB,Franz CK,Kurz J,Templer JW,Swanson GT,Horbinski C

MA1-213 was used in Immunocytochemistry to show that postoperative seizure risk in adult-type diffuse gliomas varies in large part by molecular subtype, and that IDHmut inhibitors could play a key role in mitigating such risk in IDHmut glioma patients.

Thu Jun 15 00:00:00 EDT 2023

Postoperative risk of IDH-mutant glioma-associated seizures and their potential management with IDH-mutant inhibitors.

The Journal of clinical investigation

Thu Jun 15 00:00:00 EDT 2023

Endothelial cells are an important source of BDNF in rat skeletal muscle.

Scientific reports

Cefis M,Chaney R,Quirié A,Santini C,Marie C,Garnier P,Prigent-Tessier A

Published figure using Synaptophysin monoclonal antibody (Product # MA1-213) in Immunohistochemistry (PFA fixed)

Mon Jan 10 00:00:00 EST 2022

Endothelial cells are an important source of BDNF in rat skeletal muscle.

Scientific reports

Cefis M,Chaney R,Quirié A,Santini C,Marie C,Garnier P,Prigent-Tessier A

Published figure using Synaptophysin monoclonal antibody (Product # MA1-213) in Immunohistochemistry (PFA fixed)

Mon Jan 10 00:00:00 EST 2022

Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex.

Nature communications

Wilson MN,Thunemann M,Liu X,Lu Y,Puppo F,Adams JW,Kim JH,Ramezani M,Pizzo DP,Djurovic S,Andreassen OA,Mansour AA,Gage FH,Muotri AR,Devor A,Kuzum D

MA1-213 was used in Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Western Blot to combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid.

Mon Dec 26 00:00:00 EST 2022

Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex.

Nature communications

Wilson MN,Thunemann M,Liu X,Lu Y,Puppo F,Adams JW,Kim JH,Ramezani M,Pizzo DP,Djurovic S,Andreassen OA,Mansour AA,Gage FH,Muotri AR,Devor A,Kuzum D

Mon Dec 26 00:00:00 EST 2022

Increased VLCFA-lipids and ELOVL4 underlie neurodegeneration in frontotemporal dementia.

Scientific reports

He Y,Phan K,Bhatia S,Pickford R,Fu Y,Yang Y,Hodges JR,Piguet O,Halliday GM,Kim WS

MA1-213 was used in Western Blot to investigate the role of very long chain fatty acids (VLCFA-lipids) in frontotemporal dementia.

Mon Nov 01 00:00:00 EDT 2021

Increased VLCFA-lipids and ELOVL4 underlie neurodegeneration in frontotemporal dementia.

Scientific reports

He Y,Phan K,Bhatia S,Pickford R,Fu Y,Yang Y,Hodges JR,Piguet O,Halliday GM,Kim WS

MA1-213 was used in Western Blot to investigate the role of very long chain fatty acids (VLCFA-lipids) in frontotemporal dementia.

Mon Nov 01 00:00:00 EDT 2021

Patient-Specific Retinal Organoids Recapitulate Disease Features of Late-Onset Retinitis Pigmentosa.

Frontiers in cell and developmental biology

Gao ML,Lei XL,Han F,He KW,Jin SQ,Zhang YY,Jin ZB

Published figure using Synaptophysin monoclonal antibody (Product # MA1-213) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Patient-Specific Retinal Organoids Recapitulate Disease Features of Late-Onset Retinitis Pigmentosa.

Frontiers in cell and developmental biology

Gao ML,Lei XL,Han F,He KW,Jin SQ,Zhang YY,Jin ZB

Published figure using Synaptophysin monoclonal antibody (Product # MA1-213) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: BM89 antigen; Major synaptic vesicle protein p38; Synaptophysin; Syp I

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Gene Aliases: A230093K24Rik; AI848995; MRX96; MRXSYP; p38; Syn; SYP; Syp1

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UniProt ID: (Human) P08247, (Bovine) P20488, (Rat) P07825, (Mouse) Q62277

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Entrez Gene ID: (Human) 6855, (Bovine) 280937, (Rat) 24804, (Mouse) 20977

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Function(s)

transporter activity cholesterol binding SH2 domain binding identical protein binding protein self-association protein binding GTPase binding syntaxin-1 binding protein domain specific binding protein complex binding membrane trafficking regulatory protein

Process(es)

endocytosis synaptic vesicle maturation regulation of neuronal synaptic plasticity regulation of long-term neuronal synaptic plasticity regulation of short-term neuronal synaptic plasticity synaptic vesicle membrane organization cellular response to organic substance regulation of opioid receptor signaling pathway negative regulation of exocytosis transport chemical synaptic transmission

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Synaptophysin Monoclonal Antibody (SY38)

Synaptophysin Antibody (MA1-213) in IHC (PFA)

Product Details

MA1-213

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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