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Synaptotagmin-1 Polyclonal Antibody

Synaptotagmin-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Synaptotagmin-1 Antibody (14511-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 14511-1-AP (SynAPtotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Synaptotagmin-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Synaptotagmin-1 Antibody in Western Blot (WB)

Synaptotagmin-1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14511-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synaptotagmin-1 Fusion Protein Ag5970 (77-422 aa encoded by BC058917)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

Immunogen sequence: FCICKKCLF KKKNKKKGKE KGGKNAINMK DVKDLGKTMK DQALKDDDAE TGLTDGEEKE EPKEEEKLGK LQYSLDYDFQ NNQLLVGIIQ AAELPALDMG GTSDPYVKVF LLPDKKKKFE TKVHRKTLNP VFNEQFTFKV PYSELGGKTL VMAVYDFDRF SKHDIIGEFK VPMNTVDFGH VTEEWRDLQS AEKEEQEKLG DICFSLRYVP TAGKLTVVIL EAKNLKKMDV GGLSDPYVKI HLMQNGKRLK KKKTTIKKNT LNPYYNESFS FEVPFEQIQK VQVVVTVLDY DKIGKNDAIG KVFVGYNSTG AELRHWSDML ANPRRPIAQW HTLQVEEEVD AMLAVKK (77-422 aa encoded by BC058917)

Target Information

Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus.

Cell reports

Wen L,Yang X,Wu Z,Fu S,Zhan Y,Chen Z,Bi D,Shen Y

Tue Apr 25 00:00:00 EDT 2023

Long-read sequencing and profiling of RNA-binding proteins reveals the pathogenic mechanism of aberrant splicing of an SCN1A poison exon in epilepsy.

bioRxiv : the preprint server for biology

Happ HC,Schneider PN,Hong JH,Goes E,Bandouil M,Biar CG,Ramamurthy A,Reese F,Engel K,Weckhuysen S,Scheffer IE,Mefford HC,Calhoun JD,Carvill GL

Thu May 04 00:00:00 EDT 2023

A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/Ca²⁺ Signaling Pathway.

Pharmaceuticals (Basel, Switzerland)

Liu J,Zhang C,Wang J,Huang Y,Shen D,Hu Y,Chu H,Yu X,Zhang L,Ma H

Mon Nov 28 00:00:00 EST 2022

A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/Ca²⁺ Signaling Pathway.

Pharmaceuticals (Basel, Switzerland)

Liu J,Zhang C,Wang J,Huang Y,Shen D,Hu Y,Chu H,Yu X,Zhang L,Ma H

Mon Nov 28 00:00:00 EST 2022

Discrimination of the Activity of Low-Affinity Wild-Type and High-Affinity Mutant Recombinant BoNT/B by a SIMA Cell-Based Reporter Release Assay.

Toxins

Neuschäfer-Rube F,Pathe-Neuschäfer-Rube A,Püschel GP

Mon Jan 17 00:00:00 EST 2022

ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells.

Theranostics

Pan T,Liu J,Xu S,Yu Q,Wang H,Sun H,Wu J,Zhu Y,Zhou J,Zhu Y

Wed Apr 21 00:00:00 EDT 2021

Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Sancho-Knapik S,Pastor O,Barranquero C,Herrera Marcos LV,Guillén N,Arnal C,Gascón S,Navarro MA,Rodríguez-Yoldi MJ,Busto R,Lasunción MA,Osada J

Tue Dec 01 00:00:00 EST 2020

Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.

Biochimica et biophysica acta. Molecular and cell biology of lipids

Sancho-Knapik S,Pastor O,Barranquero C,Herrera Marcos LV,Guillén N,Arnal C,Gascón S,Navarro MA,Rodríguez-Yoldi MJ,Busto R,Lasunción MA,Osada J

Tue Dec 01 00:00:00 EST 2020

Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain.

Biochimica et biophysica acta. Proteins and proteomics

Zeng HL,Yu FL,Zhang Z,Yang Q,Jin S,He X,Chen X,Shen Y,Cheng L,Guo L,Xu F

Thu Feb 01 00:00:00 EST 2018

Quantitative proteomics study of host response to virulent and attenuated pseudorabies virus infection in mouse brain.

Biochimica et biophysica acta. Proteins and proteomics

Zeng HL,Yu FL,Zhang Z,Yang Q,Jin S,He X,Chen X,Shen Y,Cheng L,Guo L,Xu F

Thu Feb 01 00:00:00 EST 2018

A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.

Frontiers in molecular neuroscience

Li Y,Wang S,Li T,Zhu L,Xu Y,Ma C

Tue Sep 29 00:00:00 EDT 2020

A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.

Frontiers in molecular neuroscience

Li Y,Wang S,Li T,Zhu L,Xu Y,Ma C

Tue Sep 29 00:00:00 EDT 2020

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Journal of proteomics

Zeng HL,Rao X,Zhang LK,Zhao X,Zhang WP,Wang J,Xu F,Guo L

Tue Sep 23 00:00:00 EDT 2014

Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.

Journal of proteomics

Zeng HL,Rao X,Zhang LK,Zhao X,Zhang WP,Wang J,Xu F,Guo L

Tue Sep 23 00:00:00 EDT 2014

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

PloS one

Tian H,Wang L,Cai R,Zheng L,Guo L

Fri Aug 28 00:00:00 EDT 2015

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

PloS one

Tian H,Wang L,Cai R,Zheng L,Guo L

Fri Aug 28 00:00:00 EDT 2015

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

PloS one

Tian H,Wang L,Cai R,Zheng L,Guo L

Fri Aug 28 00:00:00 EDT 2015

Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.

PloS one

Tian H,Wang L,Cai R,Zheng L,Guo L

Fri Aug 28 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: DKFZp781D2042; FLJ42519; p65; SV DKFZp781D2042; SV SYT; Syb1; synaptotagmin 1; synaptotagmin 2; Synaptotagmin I; Synaptotagmin-1; SYT2; SytI

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Gene Aliases: AW124717; G630098F17Rik; P65; SVP65; SYT; SYT1; SytI

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UniProt ID: (Human) P21579, (Mouse) P46096

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Entrez Gene ID: (Human) 6857, (Mouse) 20979

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Function(s)

SNARE binding phosphatidylserine binding calcium ion binding protein binding calmodulin binding calcium-dependent phospholipid binding 1-phosphatidylinositol binding phosphatidylinositol-4,5-bisphosphate binding protein C-terminus binding syntaxin-1 binding clathrin binding identical protein binding calcium-dependent protein binding low-density lipoprotein particle receptor binding phospholipid binding syntaxin binding metal ion binding membrane trafficking regulatory protein

Process(es)

detection of calcium ion vesicle fusion chemical synaptic transmission neurotransmitter secretion brain development glutamate secretion regulation of exocytosis regulation of calcium ion-dependent exocytosis positive regulation of vesicle fusion positive regulation of calcium ion-dependent exocytosis vesicle docking synaptic vesicle endocytosis calcium ion-regulated exocytosis of neurotransmitter positive regulation of synaptic transmission protein homooligomerization regulation of synaptic transmission, glutamatergic cellular response to calcium ion fast, calcium ion-dependent exocytosis of neurotransmitter regulation of regulated secretory pathway positive regulation of dendrite extension synaptic vesicle exocytosis response to calcium ion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Synaptotagmin-1 Polyclonal Antibody

Synaptotagmin-1 Antibody (14511-1-AP) in ICC/IF

Product Details

14511-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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