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Synaptotagmin 1 Antibody (OSS00022W-100UL) in IHC (P)
IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instruct... View More
Product Details
OSS00022W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from aa region 10-60 of rat Synaptotagmin 1 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The antigen is homologous in mouse.
Specificity of this antibody: Synaptotagmin 1.
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp781D2042; FLJ42519; p65; SV DKFZp781D2042; SV SYT; Syb1; synaptotagmin 1; synaptotagmin 2; Synaptotagmin I; Synaptotagmin-1; SYT2; SytI
Gene Aliases: AW124717; G630098F17Rik; P65; Syt1; SytI
UniProt ID: (Rat) P21707, (Mouse) P46096
Entrez Gene ID: (Rat) 25716, (Mouse) 20979
SNARE binding
phosphatidylserine binding
transporter activity
calcium ion binding
protein binding
calmodulin binding
phospholipid binding
calcium-dependent phospholipid binding
phosphatidylinositol-4,5-bisphosphate binding
protein C-terminus binding
syntaxin-1 binding
syntaxin binding
clathrin binding
identical protein binding
calcium-dependent protein binding
low-density lipoprotein particle receptor binding
metal ion binding
membrane trafficking regulatory protein
detection of calcium ion
neurotransmitter secretion
synaptic vesicle exocytosis
calcium ion regulated exocytosis
regulation of calcium ion-dependent exocytosis
positive regulation of vesicle fusion
positive regulation of calcium ion-dependent exocytosis
vesicle docking
positive regulation of synaptic transmission
response to calcium ion
regulation of synaptic transmission, glutamatergic
fast, calcium ion-dependent exocytosis of neurotransmitter
regulation of regulated secretory pathway
synaptic vesicle endocytosis
positive regulation of dendrite extension
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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