Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
Synaptotagmin 1 Antibody (PA5-81274) in WB
Western Blot using Synaptotagmin 1 Polyclonal Antibody (Product # PA5-81274) at 1:500 dilution. Lane A: Rat brain tissue lysate, Lane B: Mouse brain tissue lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 60 kDa. (We are unsure of the identity of these extra bands).
Product Details
PA5-81274
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human SYT1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788495
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human Synaptotagmin 1/SYT1.
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DKFZp781D2042; FLJ42519; p65; SV DKFZp781D2042; SV SYT; Syb1; synaptotagmin 2; Synaptotagmin I; Synaptotagmin-1; SYT2; SytI
Gene Aliases: P65; SVP65; SYT; SYT1
UniProt ID: (Human) P21579
Entrez Gene ID: (Human) 6857
SNARE binding
phosphatidylserine binding
calcium ion binding
protein binding
calmodulin binding
calcium-dependent phospholipid binding
1-phosphatidylinositol binding
phosphatidylinositol-4,5-bisphosphate binding
protein C-terminus binding
syntaxin-1 binding
clathrin binding
identical protein binding
calcium-dependent protein binding
low-density lipoprotein particle receptor binding
membrane trafficking regulatory protein
detection of calcium ion
vesicle fusion
chemical synaptic transmission
neurotransmitter secretion
brain development
glutamate secretion
regulation of exocytosis
regulation of calcium ion-dependent exocytosis
positive regulation of vesicle fusion
positive regulation of calcium ion-dependent exocytosis
vesicle docking
synaptic vesicle endocytosis
calcium ion-regulated exocytosis of neurotransmitter
positive regulation of synaptic transmission
protein homooligomerization
regulation of synaptic transmission, glutamatergic
cellular response to calcium ion
fast, calcium ion-dependent exocytosis of neurotransmitter
regulation of regulated secretory pathway
positive regulation of dendrite extension
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support