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Invitrogen
Synaptotagmin 1 Monoclonal Antibody (K1E005_2F9)
FIGURE: 1 / 3
Synaptotagmin 1 Antibody (MA5-55665) in IHC (P)
Immunohistochemistry analysis of Synaptotagmin 1 in paraffin-embedded cerebral cortex tissue. Sample was incubated with Synaptotagmin 1 monoclonal antibody (Product # MA5-55665) at a dilution of 1 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 3... View More
Product Details
MA5-55665
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
K1E005_2F9
Immunogen
Recombinant human SYT1 (immunogen range is 91-420).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Sequence of this protein is as follows: KKGKEKGGKN AINMKDVKDL GKTMKDQALK DDDAETGLTD GEEKEEPKEE EKLGKLQYSL DYDFQNNQLL VGIIQAAELP ALDMGGTSDP YVKVFLLPDK KKKFETKVHR KTLNPVFNEQ FTFKVPYSEL GGKTLVMAVY DFDRFSKHDI IGEFKVPMNT VDFGHVTEEW RDLQSAEKEE QEKLGDICFS LRYVPTAGKL TVVILEAKNL KKMDVGGLSD PYVKIHLMQN GKRLKKKKTT IKKNTLNPYY NESFSFEVPF EQIQKVQVVV TVLDYDKIGK NDAIGKVFVG YNSTGAELRH WSDMLANPRR PIAQWHTLQV EEEVDAMLAV
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781D2042; FLJ42519; p65; SV DKFZp781D2042; SV SYT; Syb1; synaptotagmin 1; synaptotagmin 2; Synaptotagmin I; Synaptotagmin-1; SYT2; SytI
Gene Aliases: P65; SVP65; SYT; SYT1
UniProt ID: (Human) P21579, (Rat) P21707
Entrez Gene ID: (Human) 6857, (Rat) 25716
SNARE binding
phosphatidylserine binding
calcium ion binding
protein binding
calmodulin binding
calcium-dependent phospholipid binding
1-phosphatidylinositol binding
phosphatidylinositol-4,5-bisphosphate binding
protein C-terminus binding
syntaxin-1 binding
clathrin binding
identical protein binding
calcium-dependent protein binding
low-density lipoprotein particle receptor binding
transporter activity
phospholipid binding
syntaxin binding
membrane trafficking regulatory protein
detection of calcium ion
vesicle fusion
chemical synaptic transmission
neurotransmitter secretion
brain development
glutamate secretion
regulation of exocytosis
regulation of calcium ion-dependent exocytosis
positive regulation of vesicle fusion
positive regulation of calcium ion-dependent exocytosis
vesicle docking
synaptic vesicle endocytosis
calcium ion-regulated exocytosis of neurotransmitter
positive regulation of synaptic transmission
protein homooligomerization
regulation of synaptic transmission, glutamatergic
cellular response to calcium ion
fast, calcium ion-dependent exocytosis of neurotransmitter
regulation of regulated secretory pathway
positive regulation of dendrite extension
synaptic vesicle exocytosis
calcium ion regulated exocytosis
response to calcium ion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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