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Alomone Labs, LTD
Synaptotagmin-1 (SYT1) Polyclonal Antibody
FIGURE: 1 / 2
Synaptotagmin-1 (SYT1) Antibody (ANR-003-200UL) in IHC (F)
Expression of Synaptotagmin-I in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400), followed by Anti-rabbit-Alexa-488 Antibody . Synaptotagmin-I staining (green) appears in the hilus of the hippocampal dentate gyrus. Nuclei are stained with DAPI (blue).
Product Details
ANR-003-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin 1, Intravesicular, N-terminus
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 5% sucrose, 1% BSA
Contains
0.025% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein that is widely expressed throughout the CNS and is generally thought to act as the Ca2+ sensor in the regulation of exocytosis and neurotransmitter release. Studies indicate that synaptotagmin’s Ca2+ mediated binding of SNAP25 is essential for the Ca2+ dependent triggering of membrane. It has been demonstrated that discrete residues within the c(2)b binding domain of synaptotagmin 1 independently specify endocytic rate and synaptic vesicle size. Synaptotagmin 1 may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Further, Synaptotagmin 1 binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has been reported. Synaptotagmin 1 can also bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Diseases associated with the dysfunction of Synaptotagmin 1 include food-borne botulism and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781D2042; FLJ42519; p65; SV DKFZp781D2042; SV SYT; Syb1; synaptotagmin 1; synaptotagmin 2; Synaptotagmin I; Synaptotagmin-1; SYT2; SytI
Gene Aliases: AW124717; G630098F17Rik; P65; Syt1; SytI
UniProt ID: (Rat) P21707, (Mouse) P46096
Entrez Gene ID: (Rat) 25716, (Mouse) 20979
SNARE binding
phosphatidylserine binding
transporter activity
calcium ion binding
protein binding
calmodulin binding
phospholipid binding
calcium-dependent phospholipid binding
phosphatidylinositol-4,5-bisphosphate binding
protein C-terminus binding
syntaxin-1 binding
syntaxin binding
clathrin binding
identical protein binding
calcium-dependent protein binding
low-density lipoprotein particle receptor binding
metal ion binding
membrane trafficking regulatory protein
detection of calcium ion
neurotransmitter secretion
synaptic vesicle exocytosis
calcium ion regulated exocytosis
regulation of calcium ion-dependent exocytosis
positive regulation of vesicle fusion
positive regulation of calcium ion-dependent exocytosis
vesicle docking
positive regulation of synaptic transmission
response to calcium ion
regulation of synaptic transmission, glutamatergic
fast, calcium ion-dependent exocytosis of neurotransmitter
regulation of regulated secretory pathway
synaptic vesicle endocytosis
positive regulation of dendrite extension
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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