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Invitrogen
Syrian Hamster IgG Isotype Control, PE-Cyanine5, eBioscience™
FIGURE: 1 / 1
Syrian Hamster IgG Isotype Control (15-4914-81) in Flow
C57BL/6 mouse splenocytes were stained with NK1.1 Monoclonal Antibody, eFluor 450 (Product # 48-5941-82) and staining buffer (autofluorescence) (left) or 0.125 µg of Syrian Hamster IgG Isotype Control, PE-Cyanine5 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).
Product Details
15-4914-81
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Syrian hamster
/ IgG
Recommended Isotype Control
Class
Polyclonal
Type
Isotype Control
Conjugate
Excitation/Emission Max
568/666 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_470149
Product Specific Information
Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum.
Applications Reported: This isotype control antibody has been reported for use in flow cytometric analysis.
Applications Tested: This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as the experimental antibody.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG; Immunoglobulin G; ImmunoglobulinG
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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