Invitrogen

TAGLN Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

4 References

View all (38) TAGLN antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TAGLN Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

TAGLN Antibody (PA5-29767) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of TAGLN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TAGLN Polyclonal Antibody (Product # PA5-29767) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

TAGLN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TAGLN Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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TAGLN Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across all cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of TAGLN was observed in BJ, Caco-2, HeLa, Mouse Urinary Bladder, Rat Urinary Bladder and Rat Uterus but not in Jurkat, Raji, Mouse Skeletal Muscle, Rat Skeletal Muscle and Rat Brain which are known to be low expressing (DOI: 10.1006/geno.1998.5267, 10.1371/journal.pone.0156429) using Anti-TAGLN Polyclonal Antibody (Product # PA5-29767) in Western Blot. {RE}

Product Details

PA5-29767

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 188 of Human Transgelin

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547241

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver, A-10, A-10 treated with MG132, Bovine Smooth Muscle Cell, Bovine Fibroblastic Chondrocyte.

Predicted reactivity: Mouse (97%), Rat (96%), Xenopus laevis (81%), Pig (98%), Chicken (87%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transgelin is a poor prognostic factor associated with advanced colorectal cancer (CRC) stage promoting tumor growth and migration in a TGFβ-dependent manner.

Cell death & disease

Elsafadi M,Manikandan M,Almalki S,Mahmood A,Shinwari T,Vishnubalaji R,Mobarak M,Alfayez M,Aldahmash A,Kassem M,Alajez NM

Published figure using TAGLN polyclonal antibody (Product # PA5-29767) in Western Blot

Mon May 11 00:00:00 EDT 2020

Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization.

Cell death & disease

Elsafadi M,Manikandan M,Dawud RA,Alajez NM,Hamam R,Alfayez M,Kassem M,Aldahmash A,Mahmood A

Published figure using TAGLN polyclonal antibody (Product # PA5-29767) in Immunohistochemistry

Thu Aug 04 00:00:00 EDT 2016

Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization.

Cell death & disease

Elsafadi M,Manikandan M,Dawud RA,Alajez NM,Hamam R,Alfayez M,Kassem M,Aldahmash A,Mahmood A

Published figure using TAGLN polyclonal antibody (Product # PA5-29767) in Immunohistochemistry

Thu Aug 04 00:00:00 EDT 2016

Transgelin is a TGFβ-inducible gene that regulates osteoblastic and adipogenic differentiation of human skeletal stem cells through actin cytoskeleston organization.

Cell death & disease

Elsafadi M,Manikandan M,Dawud RA,Alajez NM,Hamam R,Alfayez M,Kassem M,Aldahmash A,Mahmood A

Published figure using TAGLN polyclonal antibody (Product # PA5-29767) in Immunohistochemistry

Thu Aug 04 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 22 kDa actin-binding protein; 25 kDa F-actin-binding protein; Actin-associated protein p27; DKFZp686B01212; DKFZp686P11128; Protein WS3-10; sm22 alpha; SM22-alpha; Sm22alpha; smooth muscle 22 protein; Smooth muscle protein 22-alpha; Transgelin; transgelin variant 2

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Gene Aliases: SM22; Sm22a; SMCC; TAGLN; TAGLN1; WS3-10; Ws310

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UniProt ID: (Human) Q01995, (Bovine) Q9TS87, (Rat) P31232, (Mouse) P37804

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Entrez Gene ID: (Human) 6876, (Bovine) 513463, (Rat) 25123, (Mouse) 21345

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Function(s)

actin binding protein binding actin filament binding protein binding, bridging non-motor actin binding protein

Process(es)

muscle organ development epithelial cell differentiation cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TAGLN Polyclonal Antibody

TAGLN Antibody (PA5-29767) in ICC/IF

Product Details

PA5-29767

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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