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TARC Antibody (500-P97-1MG) in WB
Western Blot: To detect hTARC by Western Blot analysis TARC Polyclonal Antibody (Product # 500-P97-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTARC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P97-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human TARC (CCL17)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930888
Product Specific Information
AA Sequence of recombinant protein: ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TARC (CCL17). Anti-Human TARC (CCL17)-specific antibody was purified by affinity chromatography employing an immobilized Human TARC (CCL17) matrix.
Sandwich ELISA: To detect Human TARC (CCL17) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TARC (CCL17) (500-P97Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TARC (CCL17).
Western Blot: To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTARC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
TARC ligand (CCL17) belongs to the CC chemokine family. It is a small cytokine and the gene is located on q arm of chromosome 16, in humans, along with other chemokines. TARC specifically binds to T-cells and interacts with chemokine receptors CCR4 and CCR8. It plays an important role in T cell development in thymus as well as in trafficking and activation of mature T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine; C-C motif chemokine 17; CC chemokine TARC; chemokine (C-C motif) ligand 17; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; Thymus and activation-regulated chemokine
Gene Aliases: A-152E5.3; ABCD-2; CCL17; SCYA17; TARC
UniProt ID: (Human) Q92583
Entrez Gene ID: (Human) 6361
monocyte chemotaxis
chemotaxis
inflammatory response
G-protein coupled receptor signaling pathway
cell-cell signaling
multicellular organism development
neutrophil chemotaxis
positive regulation of GTPase activity
negative regulation of myoblast differentiation
lymphocyte chemotaxis
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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