Proteintech

TBX21/T-bet Polyclonal Antibody

TBX21/T-bet Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TBX21/T-bet Antibody (13700-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using (Product # 13700-1-AP) (TBX21,T-bet antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TBX21/T-bet Antibody in Western Blot (WB)

TBX21/T-bet Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13700-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TBX21,T-bet Fusion Protein Ag4618 (1-328 aa encoded by BC039739)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGIVEPGCG DMLTGTEPMP GSDEGRAPGA DPQHRYFYPE PGAQDADERR GGGSLGSPYP GGALVPAPPS RFLGAYAYPP RPQAAGFPGA GESFPPPADA EGYQPGEGYA APDPRAGLYP GPREDYALPA GLEVSGKLRV ALNNHLLWSK FNQHQTEMII TKQGRRMFPF LSFTVAGLEP TSHYRMFVDV VLVDQHHWRY QSGKWVQCGK AEGSMPGNRL YVHPDSPNTG AHWMRQEVSF GKLKLTNNKG ASNNVTQMIV LQSLHKYQPR LHIVEVNDGE PEAACNASNT HIFTFQETQF IAVTAYQNAE ITQLKIDNNP FAKGFRENF (1-328 aa encoded by BC039739 )

Target Information

T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhao Y,Zhang J,Cheng X,Huang W,Shen S,Wu S,Huang Y,Nie G,Wang H,Qiu W

Sat Jul 01 00:00:00 EDT 2023

Cisplatin Promotes the Efficacy of Immune Checkpoint Inhibitor Therapy by Inducing Ferroptosis and Activating Neutrophils.

Frontiers in pharmacology

Zhou Z,Zhao Y,Chen S,Cui G,Fu W,Li S,Lin X,Hu H

Sat Jul 16 00:00:00 EDT 2022

Network pharmacology-based study on immunomodulatory mechanism of danggui-yimucao herb pair for the treatment of RU486-induced abortion.

Journal of ethnopharmacology

Bi SJ,Huang YX,Feng LM,Yue SJ,Chen YY,Fu RJ,Xu DQ,Tang YP

Mon Jan 10 00:00:00 EST 2022

Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway.

Evidence-based complementary and alternative medicine : eCAM

Li Z,Ma D,Wang Y,Wu S,Wang L,Jiang Y,Zhang Y,Li X

Sun May 01 00:00:00 EDT 2022

Astragali Radix-Coptis Rhizoma Herb Pair Attenuates Atherosclerosis in ApoE-/- Mice by Regulating the M1/M2 and Th1/Th2 Immune Balance and Activating the STAT6 Signaling Pathway.

Evidence-based complementary and alternative medicine : eCAM

Li Z,Ma D,Wang Y,Wu S,Wang L,Jiang Y,Zhang Y,Li X

Sun May 01 00:00:00 EDT 2022

Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice.

Chinese journal of integrative medicine

Zeng ZW,Huang JQ,Chen Y,Yu X,Zhu W,Zhang DS

Fri Jul 01 00:00:00 EDT 2022

Hepatoprotective Polysaccharides from Geranium wilfordii: Purification, Structural Characterization, and Their Mechanism.

Molecules (Basel, Switzerland)

Feng JY,Xie YQ,Zhang P,Zhou Q,Khan A,Zhou ZH,Xia XS,Liu L

Fri Jun 03 00:00:00 EDT 2022

Maggot extracts promote regulatory T cell differentiation by upregulating Foxp3 in allergic rhinitis.

American journal of translational research

Liu L,Deng YQ,Jiao WE,Qiao YL,Tao ZZ,Wang Y,Hua QQ,Chen SM

Tue Jan 18 00:00:00 EST 2022

Targeting Wnt Signaling in the Tumor Immune Microenvironment to Enhancing EpCAM CAR T-Cell therapy.

Frontiers in pharmacology

Li W,Zhou Y,Wu Z,Shi Y,Tian E,Zhu Y,Wang T,Dou W,Meng X,Chen M,Zhai B,Zhu D

Fri Nov 19 00:00:00 EST 2021

Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.

Cell

Yang R,Mele F,Worley L,Langlais D,Rosain J,Benhsaien I,Elarabi H,Croft CA,Doisne JM,Zhang P,Weisshaar M,Jarrossay D,Latorre D,Shen Y,Han J,Ogishi M,Gruber C,Markle J,Al Ali F,Rahman M,Khan T,Seeleuthner Y,Kerner G,Husquin LT,Maclsaac JL,Jeljeli M,Errami A,Ailal F,Kobor MS,Oleaga-Quintas C,Roynard M,Bourgey M,El Baghdadi J,Boisson-Dupuis S,Puel A,Batteux F,Rozenberg F,Marr N,Pan-Hammarström Q,Bogunovic D,Quintana-Murci L,Carroll T,Ma CS,Abel L,Bousfiha A,Di Santo JP,Glimcher LH,Gros P,Tangye SG,Sallusto F,Bustamante J,Casanova JL

Wed Dec 23 00:00:00 EST 2020

B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters.

Infection and immunity

Johnson RM,Yu H,Strank NO,Karunakaran K,Zhu Y,Brunham RC

Thu Feb 01 00:00:00 EST 2018

B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters.

Infection and immunity

Johnson RM,Yu H,Strank NO,Karunakaran K,Zhu Y,Brunham RC

Thu Feb 01 00:00:00 EST 2018

Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Xia Y,Yu B,Ma C,Tu Y,Zhai L,Yang Y,Liu D,Liu Y,Wu H,Dan H,You P

Mon Oct 01 00:00:00 EDT 2018

Effect of IL-17 in the development of colon cancer in mice.

Oncology letters

Yang L,Liu H,Zhang L,Hu J,Chen H,Wang L,Yin X,Li Q,Qi Y

Thu Dec 01 00:00:00 EST 2016

Effect of IL-17 in the development of colon cancer in mice.

Oncology letters

Yang L,Liu H,Zhang L,Hu J,Chen H,Wang L,Yin X,Li Q,Qi Y

Thu Dec 01 00:00:00 EST 2016

CD4 T cells require ICOS-mediated PI3K signaling to increase T-Bet expression in the setting of anti-CTLA-4 therapy.

Cancer immunology research

Chen H,Fu T,Suh WK,Tsavachidou D,Wen S,Gao J,Ng Tang D,He Q,Sun J,Sharma P

Sat Feb 01 00:00:00 EST 2014

CD4 T cells require ICOS-mediated PI3K signaling to increase T-Bet expression in the setting of anti-CTLA-4 therapy.

Cancer immunology research

Chen H,Fu T,Suh WK,Tsavachidou D,Wen S,Gao J,Ng Tang D,He Q,Sun J,Sharma P

Sat Feb 01 00:00:00 EST 2014

CD4 T cells require ICOS-mediated PI3K signaling to increase T-Bet expression in the setting of anti-CTLA-4 therapy.

Cancer immunology research

Chen H,Fu T,Suh WK,Tsavachidou D,Wen S,Gao J,Ng Tang D,He Q,Sun J,Sharma P

Sat Feb 01 00:00:00 EST 2014

Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome.

International journal of cardiology

Lu Y,Li L,Yan H,Su Q,Huang J,Fu C

Tue Oct 15 00:00:00 EDT 2013

Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome.

International journal of cardiology

Lu Y,Li L,Yan H,Su Q,Huang J,Fu C

Tue Oct 15 00:00:00 EDT 2013

Inhibition of Pim-1 kinase ameliorates dextran sodium sulfate-induced colitis in mice.

Digestive diseases and sciences

Shen YM,Zhao Y,Zeng Y,Yan L,Chen BL,Leng AM,Mu YB,Zhang GY

Sun Jul 01 00:00:00 EDT 2012

Inhibition of Pim-1 kinase ameliorates dextran sodium sulfate-induced colitis in mice.

Digestive diseases and sciences

Shen YM,Zhao Y,Zeng Y,Yan L,Chen BL,Leng AM,Mu YB,Zhang GY

Sun Jul 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: HGNC:11599; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM

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Gene Aliases: T-bet; T-PET; TBET; TBLYM; TBT1; TBX21

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UniProt ID: (Human) Q9UL17, (Mouse) Q9JKD8

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Entrez Gene ID: (Human) 30009, (Mouse) 57765

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Function(s)

transcription factor activity, sequence-specific DNA binding transcription regulatory region DNA binding DNA binding protein binding Rel homology transcription factor

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development response to virus T cell differentiation positive regulation of transcription, DNA-templated positive regulation of isotype switching to IgG isotypes cellular response to organic substance lymphocyte migration regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TBX21/T-bet Polyclonal Antibody

TBX21/T-bet Antibody (13700-1-AP) in IHC (P)

Product Details

13700-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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