Proteintech

TBX3 Polyclonal Antibody

TBX3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TBX3 Antibody (16741-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using 16741-1-AP (TBX3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TBX3 Antibody in Immunoprecipitation (IP)

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Product Details

16741-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TBX3 Fusion Protein Ag9955 (397-743 aa encoded by BC025258)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TTTSEEPCR DKGSPAVKAH LFAAERPRDS GRLDKASPDS RHSPATISSS TRGLGAEERR SPVREGTAPA KVEEARALPG KEAFAPLTVQ TDAAAAHLAQ GPLPGLGFAP GLAGQQFFNG HPLFLHPSQF AMGGAFSSMA AAGMGPLLAT VSGASTGVSG LDSTAMASAA AAQGLSGASA ATLPFHLQQH VLASQGLAMS PFGSLFPYPY TYMAAAAAAS SAAASSSVHR HPFLNLNTMR PRLRYSPYSI PVPVPDGSSL LTTALPSMAA AAGPLDGKVA ALAASPASVA VDSGSELNSR SSTLSSSSMS LSPKLCAEKE AATSELQSIQ RLVSGLEAKP DRSRSASP (397-743 aa encoded by BC025258)

Target Information

T-box 3 (TBX3) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. The Tbx3 protein is a transcriptional repressor that binds the canonical Brachychury bindig sites, and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Tbx3 is required for normal mammary development and is also implicated in tumor development. Tbx3 expression also increases during osteoblast differentiation, and may function as a determinant of osteoblast cell numbers. Mutations in T-box 3 cause ulnar-mammary syndrome (MIM 181450), affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing results in three transcript variants encoding different isoforms; however, the nature of one full length variant has not been determined. Subsets of human breast cancer cell lines overexpress TBX36. in vitro, Tbx3 overexpression in mouse embryo fibroblasts leads to immortalization of cells. Truncated forms of Tbx3 are increased in plasma samples.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors.

Nature communications

Zhang P,Guan H,Yuan S,Cheng H,Zheng J,Zhang Z,Liu Y,Yu Y,Meng Z,Zheng X,Zhao L

Thu Mar 24 00:00:00 EDT 2022

Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model.

Evidence-based complementary and alternative medicine : eCAM

Yang X,Luo G,Fu L,Huang H,Wang L,Yin L,Zhang X,Wang T,Ma X,Feng T,Ye J

Sat Jul 16 00:00:00 EDT 2022

HOTAIR maintains the stemness of ovarian cancer stem cells via the miR-206/TBX3 axis.

Experimental cell research

Zhang Y,Guo J,Cai E,Cai J,Wen Y,Lu S,Li X,Han Q,Jiang J,Li T,Wang Z

Thu Oct 15 00:00:00 EDT 2020

HOTAIR maintains the stemness of ovarian cancer stem cells via the miR-206/TBX3 axis.

Experimental cell research

Zhang Y,Guo J,Cai E,Cai J,Wen Y,Lu S,Li X,Han Q,Jiang J,Li T,Wang Z

Thu Oct 15 00:00:00 EDT 2020

Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencing.

Human genomics

Xie H,Zhang E,Hong N,Fu Q,Li F,Chen S,Yu Y,Sun K

Mon Sep 17 00:00:00 EDT 2018

Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencing.

Human genomics

Xie H,Zhang E,Hong N,Fu Q,Li F,Chen S,Yu Y,Sun K

Mon Sep 17 00:00:00 EDT 2018

Leukemia inhibitory factor regulates marmoset induced pluripotent stem cell proliferation via a PI3K/‌Akt‑dependent Tbx‑3 activation pathway.

International journal of molecular medicine

Ke M,He Q,Hong D,Li O,Zhu M,Ou WB,He Y,Wu Y

Sun Jul 01 00:00:00 EDT 2018

Leukemia inhibitory factor regulates marmoset induced pluripotent stem cell proliferation via a PI3K/‌Akt‑dependent Tbx‑3 activation pathway.

International journal of molecular medicine

Ke M,He Q,Hong D,Li O,Zhu M,Ou WB,He Y,Wu Y

Sun Jul 01 00:00:00 EDT 2018

A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells.

Cellular signalling

Du HF,Ou LP,Yang X,Song XD,Fan YR,Tan B,Luo CL,Wu XH

Sat Mar 01 00:00:00 EST 2014

A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells.

Cellular signalling

Du HF,Ou LP,Yang X,Song XD,Fan YR,Tan B,Luo CL,Wu XH

Sat Mar 01 00:00:00 EST 2014

A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells.

Cellular signalling

Du HF,Ou LP,Yang X,Song XD,Fan YR,Tan B,Luo CL,Wu XH

Sat Mar 01 00:00:00 EST 2014

A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells.

Cellular signalling

Du HF,Ou LP,Yang X,Song XD,Fan YR,Tan B,Luo CL,Wu XH

Sat Mar 01 00:00:00 EST 2014

Homologous recombination efficiency enhanced by inhibition of MEK and GSK3β.

Genesis (New York, N.Y. : 2000)

Lin Z,Zhang Y,Gao T,Wang L,Zhang Q,Zhou J,Zhao J

Sat Nov 01 00:00:00 EDT 2014

Homologous recombination efficiency enhanced by inhibition of MEK and GSK3β.

Genesis (New York, N.Y. : 2000)

Lin Z,Zhang Y,Gao T,Wang L,Zhang Q,Zhou J,Zhao J

Sat Nov 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: bladder cancer related protein XHL; T-box protein 3; T-box transcription factor TBX3

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Gene Aliases: D5Ertd189e; TBX3; TBX3-ISO; UMS; XHL

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UniProt ID: (Human) O15119, (Mouse) P70324

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Entrez Gene ID: (Human) 6926, (Mouse) 21386

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding protein binding sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter skeletal system development blood vessel development in utero embryonic development heart looping outflow tract morphogenesis atrioventricular bundle cell differentiation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell aging positive regulation of cell proliferation anterior/posterior axis specification, embryo organ morphogenesis specification of organ position stem cell population maintenance limbic system development male genitalia development female genitalia development negative regulation of epithelial cell differentiation mammary gland development luteinizing hormone secretion embryonic forelimb morphogenesis embryonic hindlimb morphogenesis forelimb morphogenesis embryonic digit morphogenesis negative regulation of apoptotic process negative regulation of myoblast differentiation positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated follicle-stimulating hormone secretion mesoderm morphogenesis palate development ventricular septum morphogenesis branching involved in mammary gland duct morphogenesis mammary placode formation cardiac muscle cell fate commitment sinoatrial node cell development cellular senescence positive regulation of stem cell proliferation heart morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development embryonic heart tube development limb morphogenesis regulation of cell proliferation cardiac muscle cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TBX3 Polyclonal Antibody

TBX3 Antibody (16741-1-AP) in IHC (P)

Product Details

16741-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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