Invitrogen

TBX3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

28 Published Figures

23 References

View all (27) TBX3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TBX3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 31

TBX3 Antibody (42-4800) in IHC (P)

Immunohistochemistry analysis of TBX3 in mouse E16.5 lung sections. Antigen retrieval from 4% PFA, paraffin embedded sections was performed using EDTA. Following antigen retrieval, tissues were blocked and probed with a TBX3 polyclonal antibody (Product # 42-4800) at a dilution of 1:1000. Detection was performed using an Alexa Fluor-568-conjugated anti-rabbit secondary antibody (Product # A-11036). Data cou... View More

TBX3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

TBX3 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

TBX3 Antibody in Immunohistochemistry (IHC)

TBX3 Antibody in Western Blot, ChIP Assay (WB, ChIP)

Product Image Gallery

TBX3 Antibody — FIGURE 3: RA-mediated reduction in ME1402 cell numbers correlates with increased TBX3 levels. (A) Growth curve assay of ME1402 cells treated with vehicle or RA. Right, Western blot analysis of protein harvested from the growth curve assay. (B) Growth curve assay of ME1402 cells and shTBX3 -ME1402 cells treated with vehicle or RA. Right, Western blot analysis of protein harvested from the growth curve assay. The bar graph compares the fold reduction in ME1402 and shTBX3 cell numbers at day 9 of the growth curve assay. Bars, SD. *p < 0.05, **p < 0.001. {TM}

Product Details

42-4800

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

1:1,000

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of human T-box 3, which differs from mouse and rat by one conservative and one non-conservative amino acid

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533526

Product Specific Information

42-4800 has been successfully used in immunohistochemistry analysis of Tbx3 in mouse E16.5 frozen lung sections.

Target Information

T-box 3 (TBX3) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. The Tbx3 protein is a transcriptional repressor that binds the canonical Brachychury bindig sites, and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Tbx3 is required for normal mammary development and is also implicated in tumor development. Tbx3 expression also increases during osteoblast differentiation, and may function as a determinant of osteoblast cell numbers. Mutations in T-box 3 cause ulnar-mammary syndrome (MIM 181450), affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing results in three transcript variants encoding different isoforms; however, the nature of one full length variant has not been determined. Subsets of human breast cancer cell lines overexpress TBX36. in vitro, Tbx3 overexpression in mouse embryo fibroblasts leads to immortalization of cells. Truncated forms of Tbx3 are increased in plasma samples.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Wnt/β-catenin signaling upregulates Na_v 1.5 channels in Brugada syndrome iPSC-derived cardiomyocytes.

Physiological reports

Lu A,Gu R,Chu C,Xia Y,Wang J,Davis DR,Liang W

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Western Blot

Mon May 01 00:00:00 EDT 2023

Inhibition of Wnt/β-catenin signaling upregulates Na_v 1.5 channels in Brugada syndrome iPSC-derived cardiomyocytes.

Physiological reports

Lu A,Gu R,Chu C,Xia Y,Wang J,Davis DR,Liang W

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Western Blot

Mon May 01 00:00:00 EDT 2023

Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors.

Nature communications

Zhang P,Guan H,Yuan S,Cheng H,Zheng J,Zhang Z,Liu Y,Yu Y,Meng Z,Zheng X,Zhao L

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Western Blot

Thu Mar 24 00:00:00 EDT 2022

CREB5 reprograms FOXA1 nuclear interactions to promote resistance to androgen receptor-targeting therapies.

eLife

Hwang JH,Arafeh R,Seo JH,Baca SC,Ludwig M,Arnoff TE,Sawyer L,Richter C,Tape S,Bergom HE,McSweeney S,Rennhack JP,Klingenberg SA,Cheung ATM,Kwon J,So J,Kregel S,Van Allen EM,Drake JM,Freedman ML,Hahn WC

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Western Blot

Thu May 12 00:00:00 EDT 2022

Patterning the embryonic pulmonary mesenchyme.

iScience

Goodwin K,Jaslove JM,Tao H,Zhu M,Hopyan S,Nelson CM

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Immunohistochemistry

Fri Mar 18 00:00:00 EDT 2022

Patterning the embryonic pulmonary mesenchyme.

iScience

Goodwin K,Jaslove JM,Tao H,Zhu M,Hopyan S,Nelson CM

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Immunohistochemistry

Fri Mar 18 00:00:00 EDT 2022

Targeting the oncogenic TBX3:nucleolin complex to treat multiple sarcoma subtypes.

American journal of cancer research

Willmer T,Damerell V,Smyly S,Sims D,Du Toit M,Ncube S,Sinkala M,Govender D,Sturrock E,Blackburn JM,Prince S

42-4800 was used in Immunocytochemistry to provide the first evidence that TBX3 requires nucleolin to promote features of sarcomagenesis and that disruption of the oncogenic TBX3-nucleolin interaction by AS1411 may be a novel approach for treating sarcomas.

Wed Dec 08 00:00:00 EST 2021

Targeting the oncogenic TBX3:nucleolin complex to treat multiple sarcoma subtypes.

American journal of cancer research

Willmer T,Damerell V,Smyly S,Sims D,Du Toit M,Ncube S,Sinkala M,Govender D,Sturrock E,Blackburn JM,Prince S

Wed Dec 08 00:00:00 EST 2021

Lateral line placodes of aquatic vertebrates are evolutionarily conserved in mammals.

Biology open

Washausen S,Knabe W

42-4800 was used in Immunohistochemistry to examine how, in embryonic mice, apoptosis contributes to the remodelling of the primordial posterior placodal area into physically separated otic and epibranchial placodes.

Tue Jun 19 00:00:00 EDT 2018

DNA Methylation Changes in Tbx3 in a Mouse Model Exposed to Polybrominated Diphenyl Ethers.

International journal of toxicology

Shimbo T,Dunnick JK,Brix A,Mav D,Shah R,Roberts JD,Wade PA

42-4800 was used in Western Blotting to study the molecular mechanisms by which DE-71 influences tumour incidence or progression.

Fri Mar 02 00:00:00 EST 2018

The T-box transcription factor 3 is a promising biomarker and a key regulator of the oncogenic phenotype of a diverse range of sarcoma subtypes.

Oncogenesis

Willmer T,Cooper A,Sims D,Govender D,Prince S

42-4800 was used in western blot to study a promising biomarker and key regulator of the oncogenic phenotype of a diverse range of sarcoma subtypes called T-box transcription factor 3

Mon Feb 22 00:00:00 EST 2016

The T-box transcription factor TBX3 drives proliferation by direct repression of the p21(WAF1) cyclin-dependent kinase inhibitor.

Cell division

Willmer T,Hare S,Peres J,Prince S

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Western Blot

Mon Apr 25 00:00:00 EDT 2016

The T-box transcription factor TBX3 drives proliferation by direct repression of the p21(WAF1) cyclin-dependent kinase inhibitor.

Cell division

Willmer T,Hare S,Peres J,Prince S

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Western Blot

Mon Apr 25 00:00:00 EDT 2016

The T-Box factor TBX3 is important in S-phase and is regulated by c-Myc and cyclin A-CDK2.

Cell cycle (Georgetown, Tex.)

Willmer T,Peres J,Mowla S,Abrahams A,Prince S

42-4800 was used in Western Blotting to show that TBX3 is required for cells to transit through S-phase and that this may be linked to its role as a pro-proliferative factor.

Tue Aug 30 00:00:00 EDT 2016

The T-Box factor TBX3 is important in S-phase and is regulated by c-Myc and cyclin A-CDK2.

Cell cycle (Georgetown, Tex.)

Willmer T,Peres J,Mowla S,Abrahams A,Prince S

42-4800 was used in Western Blotting to show that TBX3 is required for cells to transit through S-phase and that this may be linked to its role as a pro-proliferative factor.

Tue Aug 30 00:00:00 EDT 2016

The T-Box factor TBX3 is important in S-phase and is regulated by c-Myc and cyclin A-CDK2.

Cell cycle (Georgetown, Tex.)

Willmer T,Peres J,Mowla S,Abrahams A,Prince S

42-4800 was used in Western Blotting to show that TBX3 is required for cells to transit through S-phase and that this may be linked to its role as a pro-proliferative factor.

Tue Aug 30 00:00:00 EDT 2016

Transcriptional repressor Tbx3 is required for the hormone-sensing cell lineage in mammary epithelium.

PloS one

Kunasegaran K,Ho V,Chang TH,De Silva D,Bakker ML,Christoffels VM,Pietersen AM

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Immunofluorescence

Fri Jun 26 00:00:00 EDT 2015

Transcriptional repressor Tbx3 is required for the hormone-sensing cell lineage in mammary epithelium.

PloS one

Kunasegaran K,Ho V,Chang TH,De Silva D,Bakker ML,Christoffels VM,Pietersen AM

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Immunofluorescence

Fri Jun 26 00:00:00 EDT 2015

Transcriptional repressor Tbx3 is required for the hormone-sensing cell lineage in mammary epithelium.

PloS one

Kunasegaran K,Ho V,Chang TH,De Silva D,Bakker ML,Christoffels VM,Pietersen AM

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Immunofluorescence

Fri Jun 26 00:00:00 EDT 2015

Transcriptional repressor Tbx3 is required for the hormone-sensing cell lineage in mammary epithelium.

PloS one

Kunasegaran K,Ho V,Chang TH,De Silva D,Bakker ML,Christoffels VM,Pietersen AM

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Immunofluorescence

Fri Jun 26 00:00:00 EDT 2015

The anti-proliferative function of the TGF-β1 signaling pathway involves the repression of the oncogenic TBX2 by its homologue TBX3.

The Journal of biological chemistry

Li J,Ballim D,Rodriguez M,Cui R,Goding CR,Teng H,Prince S

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Western Blot

Fri Dec 19 00:00:00 EST 2014

The anti-proliferative function of the TGF-β1 signaling pathway involves the repression of the oncogenic TBX2 by its homologue TBX3.

The Journal of biological chemistry

Li J,Ballim D,Rodriguez M,Cui R,Goding CR,Teng H,Prince S

Published figure using TBX3 polyclonal antibody (Product # 42-4800) in Western Blot

Fri Dec 19 00:00:00 EST 2014

TBX3 Directs Cell-Fate Decision toward Mesendoderm.

Stem cell reports

Weidgang CE,Russell R,Tata PR,Kühl SJ,Illing A,Müller M,Lin Q,Brunner C,Boeckers TM,Bauer K,Kartikasari AE,Guo Y,Radenz M,Bernemann C,Weiß M,Seufferlein T,Zenke M,Iacovino M,Kyba M,Schöler HR,Kühl M,Liebau S,Kleger A

424800 was used in ChIP assay to report that TBX3 is an upstream regulator of the mesendoderm transcriptional program during gastrulation

Mon May 11 00:00:00 EDT 2015

The oncogenic TBX3 is a downstream target and mediator of the TGF-β1 signaling pathway.

Molecular biology of the cell

Li J,Weinberg MS,Zerbini L,Prince S

42-4800 was used in western blot to identify the TGF-β signaling pathway as a regulator of TBX3 in development and cancer.

Fri Nov 01 00:00:00 EDT 2013

The oncogenic TBX3 is a downstream target and mediator of the TGF-β1 signaling pathway.

Molecular biology of the cell

Li J,Weinberg MS,Zerbini L,Prince S

42-4800 was used in western blot to identify the TGF-β signaling pathway as a regulator of TBX3 in development and cancer.

Fri Nov 01 00:00:00 EDT 2013

The ulnar-mammary syndrome gene, Tbx3, is a direct target of the retinoic acid signaling pathway, which regulates its expression during mouse limb development.

Molecular biology of the cell

Ballim RD,Mendelsohn C,Papaioannou VE,Prince S

42-4800 was used in western blot to investigate how retinoic acid activates endogenous TBX3 expression.

Fri Jun 01 00:00:00 EDT 2012

Basal transcription of the human TBX3 gene, a key developmental regulator which is overexpressed in several cancers, requires functional NF-Y and Sp1 sites.

Gene

Smith J,Mowla S,Prince S

42-4800 was used in western blot to elucidate the mechanisms regulating TBX3 expression.

Sat Oct 15 00:00:00 EDT 2011

PMA-induced up-regulation of TBX3 is mediated by AP-1 and contributes to breast cancer cell migration.

The Biochemical journal

Mowla S,Pinnock R,Leaner VD,Goding CR,Prince S

42-4800 was used in western blot to study the regulation of the TBX3 gene

Sat Jan 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: bladder cancer related protein XHL; T-box 3 (ulnar mammary syndrome); T-box protein 3; T-box transcription factor TBX3

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Gene Aliases: D5Ertd189e; TBX3; TBX3-ISO; UMS; XHL

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UniProt ID: (Human) O15119, (Mouse) P70324, (Rat) Q7TST9

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Entrez Gene ID: (Human) 6926, (Mouse) 21386, (Rat) 353305

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding protein binding sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding molecular_function Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter skeletal system development blood vessel development in utero embryonic development heart looping outflow tract morphogenesis atrioventricular bundle cell differentiation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell aging positive regulation of cell proliferation anterior/posterior axis specification, embryo organ morphogenesis specification of organ position stem cell population maintenance limbic system development male genitalia development female genitalia development negative regulation of epithelial cell differentiation mammary gland development luteinizing hormone secretion embryonic forelimb morphogenesis embryonic hindlimb morphogenesis forelimb morphogenesis embryonic digit morphogenesis negative regulation of apoptotic process negative regulation of myoblast differentiation positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated follicle-stimulating hormone secretion mesoderm morphogenesis palate development ventricular septum morphogenesis branching involved in mammary gland duct morphogenesis mammary placode formation cardiac muscle cell fate commitment sinoatrial node cell development cellular senescence positive regulation of stem cell proliferation heart morphogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development embryonic heart tube development limb morphogenesis regulation of cell proliferation cardiac muscle cell differentiation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TBX3 Polyclonal Antibody

TBX3 Antibody (42-4800) in IHC (P)

Product Details

42-4800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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