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TBX3 Antibody (PA5-148438) in ICC/IF
Immunocytochemistry analysis of TBX3 in Hela cells. Samples were incubated with TBX3 polyclonal antibody (Product # PA5-148438) using a dilution of 1:100 at 4° overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, and blocked with 10% serum. Counterstained with DAPI (blue). Positive staining was localized to Nucleus and Cytoplasm.
Product Details
PA5-148438
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived Human TBX3 fragment
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7
Contains
0.03% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
T-box 3 (TBX3) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. The Tbx3 protein is a transcriptional repressor that binds the canonical Brachychury bindig sites, and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Tbx3 is required for normal mammary development and is also implicated in tumor development. Tbx3 expression also increases during osteoblast differentiation, and may function as a determinant of osteoblast cell numbers. Mutations in T-box 3 cause ulnar-mammary syndrome (MIM 181450), affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing results in three transcript variants encoding different isoforms; however, the nature of one full length variant has not been determined. Subsets of human breast cancer cell lines overexpress TBX36. in vitro, Tbx3 overexpression in mouse embryo fibroblasts leads to immortalization of cells. Truncated forms of Tbx3 are increased in plasma samples.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bladder cancer related protein XHL; T-box protein 3; T-box transcription factor TBX3
Gene Aliases: TBX3; TBX3-ISO; UMS; XHL
UniProt ID: (Human) O15119
Entrez Gene ID: (Human) 6926
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
RNA polymerase II activating transcription factor binding
protein binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
blood vessel development
in utero embryonic development
heart looping
outflow tract morphogenesis
atrioventricular bundle cell differentiation
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell aging
positive regulation of cell proliferation
anterior/posterior axis specification, embryo
organ morphogenesis
specification of organ position
stem cell population maintenance
limbic system development
male genitalia development
female genitalia development
negative regulation of epithelial cell differentiation
mammary gland development
luteinizing hormone secretion
embryonic forelimb morphogenesis
embryonic hindlimb morphogenesis
forelimb morphogenesis
embryonic digit morphogenesis
negative regulation of apoptotic process
negative regulation of myoblast differentiation
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
follicle-stimulating hormone secretion
mesoderm morphogenesis
palate development
ventricular septum morphogenesis
branching involved in mammary gland duct morphogenesis
mammary placode formation
cardiac muscle cell fate commitment
sinoatrial node cell development
cellular senescence
positive regulation of stem cell proliferation
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