Invitrogen

TBX3 Recombinant Superclonal Antibody (22HCLC)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure
1 Reference
View all (27) TBX3 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

711087

Applications
Tested Dilution
Publications

Western Blot (WB)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL
-

ChIP assay (ChIP)

2.5 µg x 10^6 cells
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

22HCLC

Immunogen

Protein corresponding to Human TBX3 (aa 305-500)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633115

Product Specific Information

This antibody is predicted to react with Monkey, Pig and Rat

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

T-box 3 (TBX3) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. The Tbx3 protein is a transcriptional repressor that binds the canonical Brachychury bindig sites, and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Tbx3 is required for normal mammary development and is also implicated in tumor development. Tbx3 expression also increases during osteoblast differentiation, and may function as a determinant of osteoblast cell numbers. Mutations in T-box 3 cause ulnar-mammary syndrome (MIM 181450), affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing results in three transcript variants encoding different isoforms; however, the nature of one full length variant has not been determined. Subsets of human breast cancer cell lines overexpress TBX36. in vitro, Tbx3 overexpression in mouse embryo fibroblasts leads to immortalization of cells. Truncated forms of Tbx3 are increased in plasma samples.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bladder cancer related protein XHL; T-box protein 3; T-box transcription factor TBX3

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Gene Aliases: TBX3; TBX3-ISO; UMS; XHL

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UniProt ID: (Human) O15119

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Entrez Gene ID: (Human) 6926

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding protein binding sequence-specific DNA binding Rel homology transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter skeletal system development blood vessel development in utero embryonic development heart looping outflow tract morphogenesis atrioventricular bundle cell differentiation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell aging positive regulation of cell proliferation anterior/posterior axis specification, embryo organ morphogenesis specification of organ position stem cell population maintenance limbic system development male genitalia development female genitalia development negative regulation of epithelial cell differentiation mammary gland development luteinizing hormone secretion embryonic forelimb morphogenesis embryonic hindlimb morphogenesis forelimb morphogenesis embryonic digit morphogenesis negative regulation of apoptotic process negative regulation of myoblast differentiation positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated follicle-stimulating hormone secretion mesoderm morphogenesis palate development ventricular septum morphogenesis branching involved in mammary gland duct morphogenesis mammary placode formation cardiac muscle cell fate commitment sinoatrial node cell development cellular senescence positive regulation of stem cell proliferation
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