Invitrogen

TCR V alpha 2 Monoclonal Antibody (B20.1), PE, eBioscience™

3 Published Figures

21 References

View all (18) TCR V alpha 2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (3)

TCR V alpha 2 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

TCR V alpha 2 Antibody (12-5812-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and Anti-Mouse CD8a FITC (Product # 11-0081-82) and 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Mouse V alpha 2 TCR PE (right). Total viable cells were used for analysis.

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Product Details

12-5812-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 20 publications 20 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B20.1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465949

Product Specific Information

Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology.

Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (Product # 14-0161-85). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Mechanisms of CD40-dependent cDC1 licensing beyond costimulation.

Nature immunology

Wu R,Ohara RA,Jo S,Liu TT,Ferris ST,Ou F,Kim S,Theisen DJ,Anderson DA,Wong BW,Gershon T,Schreiber RD,Murphy TL,Murphy KM

12-5812-82 was used in Flow cytometry/Cell sorting to identify CD40-induced genes in cDC1s, including Cd70, Tnfsf9, Ptgs2 and Bcl2l1, and examined their contributions to anti-tumor immunity.

Tue Nov 01 00:00:00 EDT 2022

Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4.

Nature

Campisi L,Chizari S,Ho JSY,Gromova A,Arnold FJ,Mosca L,Mei X,Fstkchyan Y,Torre D,Beharry C,Garcia-Forn M,Jiménez-Alcázar M,Korobeynikov VA,Prazich J,Fayad ZA,Seldin MM,De Rubeis S,Bennett CL,Ostrow LW,Lunetta C,Squatrito M,Byun M,Shneider NA,Jiang N,La Spada AR,Marazzi I

12-5812-82 was used in Flow cytometry/Cell sorting to provide evidence of an antigen-specific CD8 T cell response in ALS4, which could be used to unravel disease mechanisms and as a potential biomarker of disease state.

Wed Jun 01 00:00:00 EDT 2022

Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.

Protein & cell

Tong D,Zhang L,Ning F,Xu Y,Hu X,Shi Y

12-5812-82 was used in Flow Cytometry to report here that resting, previously antigen-stimulated CD4 T cells maintain a minimalist response to dendritic cells after their peak activation in vitro.

Sat Feb 01 00:00:00 EST 2020

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

12-5812 was used in Flow cytometry/Cell sorting to show homeostasis of thymically-derived and peripherally-derived Treg cells is maintained through the balancing of TCR signaling induced transcriptional activity by Bach2.

Tue Jan 14 00:00:00 EST 2020

The Bone Marrow Protects and Optimizes Immunological Memory during Dietary Restriction.

Cell

Collins N,Han SJ,Enamorado M,Link VM,Huang B,Moseman EA,Kishton RJ,Shannon JP,Dixit D,Schwab SR,Hickman HD,Restifo NP,McGavern DB,Schwartzberg PL,Belkaid Y

12-5812-82 was used in Flow Cytometry to show that memory T cells collapsed in secondary lymphoid organs in the context of dietary restriction (DR) but dramatically accumulated within the bone marrow (BM), where they adopted a state associated with energy conservation.

Thu Aug 22 00:00:00 EDT 2019

GM-CSF Promotes Antitumor Immunity by Inducing Th9 Cell Responses.

Cancer immunology research

Kim IK,Koh CH,Jeon I,Shin KS,Kang TS,Bae EA,Seo H,Ko HJ,Kim BS,Chung Y,Kang CY

12-5812-82 was used in Flow Cytometry to demonstrate that GM-CSF potentiates the efficacy of cancer vaccines through IL9-producing Th (Th9) cells.

Fri Mar 01 00:00:00 EST 2019

Expression of the Phosphatase Ppef2 Controls Survival and Function of CD8⁺ Dendritic Cells.

Frontiers in immunology

Zwick M,Ulas T,Cho YL,Ried C,Grosse L,Simon C,Bernhard C,Busch DH,Schultze JL,Buchholz VR,Stutte S,Brocker T

12-5812-82 was used in Flow Cytometry to suggest that Ppef2 is crucial to support survival of immature CD8+ DCs, while Ppef2 down-regulation during DC-maturation limits T cell responses.

Tue Oct 27 00:00:00 EDT 2020

Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8⁺ T Cells.

Immunity

Li J,Lee Y,Li Y,Jiang Y,Lu H,Zang W,Zhao X,Liu L,Chen Y,Tan H,Yang Z,Zhang MQ,Mak TW,Ni L,Dong C

12-5812 was used in Flow cytometry/Cell sorting to demonstrate that increased B7S1 expression in myeloid cells from human hepatocellular carcinoma is correlated with CD8+ T cell dysfunction.

Tue Apr 17 00:00:00 EDT 2018

Altered compensatory cytokine signaling underlies the discrepancy between and mice.

The Journal of experimental medicine

Durai V,Bagadia P,Briseño CG,Theisen DJ,Iwata A,Davidson JT,Gargaro M,Fremont DH,Murphy TL,Murphy KM

12-5812 was used in Flow cytometry/Cell sorting to determine the role of Flt3L in dendritic cell development.

Mon May 07 00:00:00 EDT 2018

Store-Operated Ca²⁺ Entry Controls Clonal Expansion of T Cells through Metabolic Reprogramming.

Immunity

Vaeth M,Maus M,Klein-Hessling S,Freinkman E,Yang J,Eckstein M,Cameron S,Turvey SE,Serfling E,Berberich-Siebelt F,Possemato R,Feske S

Tue Oct 17 00:00:00 EDT 2017

DC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo.

The Journal of experimental medicine

Lehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D

12-5812 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice.

Mon May 01 00:00:00 EDT 2017

Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses.

Immunology

Xu Y,Liu Y,Yang C,Kang L,Wang M,Hu J,He H,Song W,Tang H

Published figure using TCR V alpha 2 monoclonal antibody (Product # 12-5812-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

Low-Affinity Memory CD8+ T Cells Mediate Robust Heterologous Immunity.

Journal of immunology (Baltimore, Md. : 1950)

Krummey SM,Martinez RJ,Andargachew R,Liu D,Wagener M,Kohlmeier JE,Evavold BD,Larsen CP,Ford ML

12-5812 was used in Binding experiments to investigate the impact of TCR-priming affinity on memory T cell populations following a graft rechallenge.

Tue Mar 15 00:00:00 EDT 2016

Biphasic function of TLR3 adjuvant on tumor and spleen dendritic cells promotes tumor T cell infiltration and regression in a vaccine therapy.

Oncoimmunology

Azuma M,Takeda Y,Nakajima H,Sugiyama H,Ebihara T,Oshiumi H,Matsumoto M,Seya T

12-5812 was used in Flow cytometry/Cell sorting to study the effects of Poly(I:C) adjuvant therapy on the maturation of CD8α(+) dendritic cells to establish TLR3-mediated IL-12 function and cross-presentation in the spleen and lymphocyte-attractive anti-tumour microenvironment.

Mon Aug 01 00:00:00 EDT 2016

Ontogeny and localization of the cells produce IL-2 in healthy animals.

Cytokine

Yamamoto M,Seki Y,Iwai K,Ko I,Martin A,Tsuji N,Miyagawa S,Love RB,Iwashima M

12-5812 was used in Flow cytometry/Cell sorting to study how a group of Tr1 cells may have additional roles in immune homeostasis through IL-2 production to aid in the maintenance of other regulatory T cells..

Fri Mar 01 00:00:00 EST 2013

Normal T cell selection occurs in CD205-deficient thymic microenvironments.

PloS one

Jenkinson WE,Nakamura K,White AJ,Jenkinson EJ,Anderson G

Published figure using TCR V alpha 2 monoclonal antibody (Product # 12-5812-82) in Flow Cytometry

Mon Jun 24 00:00:00 EDT 2013

Novel murine dendritic cell lines: a powerful auxiliary tool for dendritic cell research.

Frontiers in immunology

Fuertes Marraco SA,Grosjean F,Duval A,Rosa M,Lavanchy C,Ashok D,Haller S,Otten LA,Steiner QG,Descombes P,Luber CA,Meissner F,Mann M,Szeles L,Reith W,Acha-Orbea H

12-5812 was used in Flow cytometry/Cell sorting to report the generation of new murine DC lines, named MutuDC, originating from cultures of splenic CD8α conventional DC (cDC) tumours.

Tue Nov 20 00:00:00 EST 2012

IL-6 induced by Staphylococcus aureus infection prevents the induction of skin allograft acceptance in mice.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Ahmed EB,Wang T,Daniels M,Alegre ML,Chong AS

12-5812 was used in Flow cytometry/Cell sorting to reveal that infection with S. aureus, but not P. aeruginosa, at the time of transplantation prohibits successful skin allograft acceptance by inducing inflammation and IL-6 production.

Sun May 01 00:00:00 EDT 2011

One naive T cell, multiple fates in CD8+ T cell differentiation.

The Journal of experimental medicine

Gerlach C,van Heijst JW,Swart E,Sie D,Armstrong N,Kerkhoven RM,Zehn D,Bevan MJ,Schepers K,Schumacher TN

12-5812 was used in Flow cytometry/Cell sorting to assess the potential for development of single naive T cells into different subsets.

Mon Jun 07 00:00:00 EDT 2010

Cell-cell cooperation at the T helper cell/mast cell immunological synapse.

Blood

Gaudenzio N,Espagnolle N,Mars LT,Liblau R,Valitutti S,Espinosa E

12-5812 was used in Flow cytometry/Cell sorting to investigate whether cognate interactions between mast cells and class II-restricted CD4(+) T cells occur.

Thu Dec 03 00:00:00 EST 2009

Engagement of TLR2 does not reverse the suppressor function of mouse regulatory T cells, but promotes their survival.

Journal of immunology (Baltimore, Md. : 1950)

Chen Q,Davidson TS,Huter EN,Shevach EM

12-5812 was used in Flow cytometry/Cell sorting to investigate the role of TLRs in adaptive immunity and in regulatory T cells.

Thu Oct 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: TCRVA; TCRVA2; V alpha 2; V alpha-2 TCR; Va 2; Va2; Valpha 2; Valpha2

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TCR V alpha 2 Monoclonal Antibody (B20.1), PE, eBioscience™

TCR V alpha 2 Antibody (12-5812-82) in Flow

Product Details

12-5812-82

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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