Invitrogen

TCR V alpha 2 Monoclonal Antibody (B20.1), Biotin, eBioscience™

TCR V alpha 2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

TCR V alpha 2 Antibody (13-5812-82) in ICC/IF

Oncoimmunology 2016 - Published figure using TCR V alpha 2 monoclonal antibody (Product # 13-5812-82) in Immunocytochemistry

TCR V alpha 2 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

13-5812-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B20.1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466687

Product Specific Information

Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology.

Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis and immunoprecipitation.

Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (Product # 14-0161-85). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Engulfment of mast cell secretory granules on skin inflammation boosts dendritic cell migration and priming efficiency.

The Journal of allergy and clinical immunology

Dudeck J,Froebel J,Kotrba J,Lehmann CHK,Dudziak D,Speier S,Nedospasov SA,Schraven B,Dudeck A

13-5812 was used in Flow cytometry/Cell sorting to show peripheral mast cells can modify dendritic cell functionality to influence lymphoid tissue-borne adaptive immunity.

Wed May 01 00:00:00 EDT 2019

Critical Role for the Microbiota in CX₃CR1⁺ Intestinal Mononuclear Phagocyte Regulation of Intestinal T Cell Responses.

Immunity

Kim M,Galan C,Hill AA,Wu WJ,Fehlner-Peach H,Song HW,Schady D,Bettini ML,Simpson KW,Longman RS,Littman DR,Diehl GE

13-5812 was used in Flow cytometry/Cell sorting to identify a cellular mechanism by which the microbiota limits intestinal inflammation and promotes tissue homeostasis.

Tue Jul 17 00:00:00 EDT 2018

DC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo.

The Journal of experimental medicine

Lehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D

13-5812 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice.

Mon May 01 00:00:00 EDT 2017

Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses.

Immunology

Xu Y,Liu Y,Yang C,Kang L,Wang M,Hu J,He H,Song W,Tang H

Published figure using TCR V alpha 2 monoclonal antibody (Product # 13-5812-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

Biphasic function of TLR3 adjuvant on tumor and spleen dendritic cells promotes tumor T cell infiltration and regression in a vaccine therapy.

Oncoimmunology

Azuma M,Takeda Y,Nakajima H,Sugiyama H,Ebihara T,Oshiumi H,Matsumoto M,Seya T

Published figure using TCR V alpha 2 monoclonal antibody (Product # 13-5812-82) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

Nuclear export of histone deacetylase 7 during thymic selection is required for immune self-tolerance.

The EMBO journal

Kasler HG,Lim HW,Mottet D,Collins AM,Lee IS,Verdin E

13-5812 was used in Flow cytometry/Cell sorting to examine the effect of blocking TCR-dependent nuclear export of HDAC7 during thymic selection, showing that HDAC7 is required for immune self-tolerance.

Wed Nov 28 00:00:00 EST 2012

Normal T cell selection occurs in CD205-deficient thymic microenvironments.

PloS one

Jenkinson WE,Nakamura K,White AJ,Jenkinson EJ,Anderson G