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TCR gamma/delta Monoclonal Antibody (5A6.E9), FITC

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

14 References

View all (45) TCR gamma/delta antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
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TCR gamma/delta Antibody in Flow Cytometry (Flow)

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TCR gamma/delta Antibody (TCR2061) in Flow

Flow cytometry analysis of TCR gamma/delta on TCR gamma/delta positive PEER cells or TCR gamma/delta negative Jurkat cells. Equal numbers of cells were stained with a FITC-labeled TCR gamma + delta (5A6.E9) monoclonal antibody (Product # TCR2061) or were left unstained (black histograms). 5 µL of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.

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Product Details

TCR2061

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1-2 µg/test

View 4 publications 4 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5A6.E9

Immunogen

Pan TCR gamma delta

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 0.5% BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223620

Product Specific Information

TCR2061 targets TCR Pan TCR gamma delta in FACS applications and shows reactivity with Human samples.

The TCR2061 immunogen is pan TCR gamma delta.

TCR2061 detects TCR Pan TCR gamma delta which has a predicted molecular weight of approximately 18 kDa.

Target Information

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Generation of MR1-Restricted T Cell Clones by Limiting Dilution Cloning of MR1 Tetramer⁺ Cells.

Methods in molecular biology (Clifton, N.J.)

Cansler M,Null M,Meermeier E,Swarbrick G,Lewinsohn D,Harriff M

TCR2061 was used in Flow cytometry/Cell sorting to develop a modifiable method for sorting and cloning MR1-restricted T cells from any tissue.

Mon Jan 18 00:00:00 EST 2021

Nonhepatosplenic γδ T-cell lymphomas represent a spectrum of aggressive cytotoxic T-cell lymphomas with a mainly extranodal presentation.

The American journal of surgical pathology

Garcia-Herrera A,Song JY,Chuang SS,Villamor N,Colomo L,Pittaluga S,Alvaro T,Rozman M,de Anda Gonzalez J,Arrunategui AM,Fernandez E,Gonzalvo E,Estrach T,Colomer D,Raffeld M,Gaulard P,Campo E,Jaffe ES,Martinez A

TCR2061 was used in immunohistochemistry to investigate the clinical heterogeneity of nonhepatosplenic gamma-delta T-cell lymphomas

Mon Aug 01 00:00:00 EDT 2011

Human mucosal associated invariant T cells detect bacterially infected cells.

PLoS biology

Gold MC,Cerri S,Smyk-Pearson S,Cansler ME,Vogt TM,Delepine J,Winata E,Swarbrick GM,Chua WJ,Yu YY,Lantz O,Cook MS,Null MD,Jacoby DB,Harriff MJ,Lewinsohn DA,Hansen TH,Lewinsohn DM

TCR2061 was used in blocking or activating experiment to investigate the role of nonclassically restricted CD8+ T cells during Mycobacterium tuberculosis infection

Tue Jun 29 00:00:00 EDT 2010

Human placenta expresses and secretes NKG2D ligands via exosomes that down-modulate the cognate receptor expression: evidence for immunosuppressive function.

Journal of immunology (Baltimore, Md. : 1950)

Hedlund M,Stenqvist AC,Nagaeva O,Kjellberg L,Wulff M,Baranov V,Mincheva-Nilsson L

TCR2061 was used in flow cytometry to investigate the role of NKG2D ligand-bearing placental exosomes in human placenta

Wed Jul 01 00:00:00 EDT 2009

IL-23/IL-17 immunity as a hallmark of Crohn's disease.

Inflammatory bowel diseases

Hölttä V,Klemetti P,Sipponen T,Westerholm-Ormio M,Kociubinski G,Salo H,Räsänen L,Kolho KL,Färkkilä M,Savilahti E,Vaarala O

TCR2061 was used in immunohistochemistry to study IL-23/IL-17 immunity as a hallmark of Crohn's disease

Mon Sep 01 00:00:00 EDT 2008

Human CD1-restricted T cell recognition of lipids from pollens.

The Journal of experimental medicine

Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F

TCR2061 was used in flow cytometry to study the immune response of pollen lipids

Mon Jul 18 00:00:00 EDT 2005

Clinicopathologic analysis of 22 cases of subcutaneous panniculitis-like CD56- or CD56+ lymphoma and review of 44 other reported cases.

American journal of clinical pathology

Takeshita M,Imayama S,Oshiro Y,Kurihara K,Okamoto S,Matsuki Y,Nakashima Y,Okamura T,Shiratsuchi M,Hayashi T,Kikuchi M

TCR2061 was used in immunohistochemistry to study subcutaneous panniculitis-like CD56- or CD56+ lymphoma during labor

Mon Mar 01 00:00:00 EST 2004

Flexible migration program regulates gamma delta T-cell involvement in humoral immunity.

Blood

Brandes M,Willimann K,Lang AB,Nam KH,Jin C,Brenner MB,Morita CT,Moser B

TCR2061 was used in immunohistochemistry to investigate the migration of gamma delta T cells

Sat Nov 15 00:00:00 EST 2003

Demographic and immune correlates of human herpesvirus 8 seropositivity in Malawi, Africa.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

DeSantis SM,Pau CP,Archibald LK,Nwanyanwu OC,Kazembe PN,Dobbie H,Jarvis WR,Jason J

TCR2061 was used in ELISA to investigate the status of human herpesvirus 8 seropositivity in Malawians

Sun Dec 01 00:00:00 EST 2002

Unaltered distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Korhonen M,Ormio M,Burgeson RE,Virtanen I,Savilahti E

TCR2061 was used in immunohistochemistry to investigate the expression and distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa

Sat Jul 01 00:00:00 EDT 2000

Small bowel T cells, HLA class II antigen DR, and GroEL stress protein in IgA nephropathy.

Kidney international

Rantala I,Collin P,Holm K,Kainulainen H,Mustonen J,Mäki M

TCR2061 was used in immunohistochemistry to study the roles of intestinal mucosa in the pathogenesis of IgA nephropathy

Tue Jun 01 00:00:00 EDT 1999

Restricted usage of T cell receptor V alpha-V beta genes in infiltrating cells in the hearts of patients with acute myocarditis and dilated cardiomyopathy.

The Journal of clinical investigation

Seko Y,Ishiyama S,Nishikawa T,Kasajima T,Hiroe M,Kagawa N,Osada K,Suzuki S,Yagita H,Okumura K

TCR2061 was used in immunohistochemistry to study the mechanism for the pathogenesis of dilated cardiomyopathy

Tue Aug 01 00:00:00 EDT 1995

Are gamma delta T cells important for the elimination of virus-infected cells?

Journal of medical primatology

Malkovsky M,Bartz SR,MacKenzie D,Radtke BE,Wallace M,Manning J,Pauza CD,Fisch P

TCR2061 was used in flow cytometry to investigate the role of gamma delta T cells in the killing of virus-infected cells

Tue Dec 01 00:00:00 EST 1992

Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease.

Scandinavian journal of immunology

Halstensen TS,Scott H,Brandtzaeg P

TCR2061 was used in immunocytochemistry to investigate the changes of TCR gamma/delta+ CD8- T cells in patients with coeliac disease

Fri Dec 01 00:00:00 EST 1989

Bioinformatics

Protein Aliases: T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex

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TCR gamma/delta Monoclonal Antibody (5A6.E9), FITC

TCR gamma/delta Antibody (TCR2061) in Flow

Product Details

TCR2061

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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