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TCTN3 Antibody (PA5-102322) in ICC/IF
Immunofluorescent analysis of TCTN3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TCTN3 polyclonal antibody (Product # PA5-102322) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102322
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TCTN3(Accession Q6NUS6), corresponding to amino acid residues T181-A231.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851730
Product Specific Information
Antibody detects endogenous levels of total TCTN3.
Target Information
TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RP11-7D5.3; tectonic 3; tectonic family member 3; Tectonic-3
Gene Aliases: 4930521E07Rik; AI197391; C10orf61; JBTS18; OFD4; PSEC0041; RGD1305166; TCTN3; TECT3; UNQ1881/PRO4324
UniProt ID: (Human) Q6NUS6, (Mouse) Q8R2Q6
Entrez Gene ID: (Human) 26123, (Mouse) 67590, (Rat) 309486
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