Proteintech

TDP-43 Polyclonal Antibody

TDP-43 Antibody in Immunocytochemistry (ICC/IF)

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TDP-43 Antibody (18280-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 18280-1-AP (TDP-43 (full length) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

TDP-43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TDP-43 Antibody in Flow Cytometry (Flow)

TDP-43 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

18280-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length TDP-43 Fusion Protein Ag13118

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.

Target Information

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Seeding the aggregation of TDP-43 requires post-fibrillization proteolytic cleavage.

Nature neuroscience

Kumar ST,Nazarov S,Porta S,Maharjan N,Cendrowska U,Kabani M,Finamore F,Xu Y,Lee VM,Lashuel HA

Thu Jun 01 00:00:00 EDT 2023

Design and application of a customizable relational DataBase to assess clinicopathological correlations and concomitant pathology in neurodegenerative diseases.

Brain pathology (Zurich, Switzerland)

Journe-Mallet I,Gouju J,Etcharry-Bouyx F,Chauvire V,Guillet-Pichon V,Scherer-Gagou C,Prundean A,Godard S,Lecluse A,Cassereau J,Verny C,Letournel F,Codron P

Mon May 01 00:00:00 EDT 2023

An in situ and in vitro investigation of cytoplasmic TDP-43 inclusions reveals the absence of a clear amyloid signature.

Annals of medicine

Cascella R,Banchelli M,Abolghasem Ghadami S,Ami D,Gagliani MC,Bigi A,Staderini T,Tampellini D,Cortese K,Cecchi C,Natalello A,Adibi H,Matteini P,Chiti F

Fri Dec 01 00:00:00 EST 2023

Multi-Modal Biomarkers of Repetitive Head Impacts and Traumatic Encephalopathy Syndrome: A Clinicopathological Case Series.

Journal of neurotrauma

Asken BM,Tanner JA,VandeVrede L,Casaletto KB,Staffaroni AM,Mundada N,Fonseca C,Iaccarino L,La Joie R,Tsuei T,Mladinov M,Grant H,Shankar R,Wang KKW,Xu H,Cobigo Y,Rosen H,Gardner RC,Perry DC,Miller BL,Spina S,Seeley WW,Kramer JH,Grinberg LT,Rabinovici GD

Thu Sep 01 00:00:00 EDT 2022

Pathological Appearance of a Case of Preclinical Multiple System Atrophy: A Comparison With Advanced Cases.

Journal of neuropathology and experimental neurology

Okada K,Hata Y,Ichimata S,Yoshida K,Nishida N

Wed Nov 16 00:00:00 EST 2022

First Family of MATR3-Related Distal Myopathy From Italy: The Role of Muscle Biopsy in the Diagnosis and Characterization of a Still Poorly Understood Disease.

Frontiers in neurology

Cavalli M,Cardani R,Renna LV,Toffetti M,Villa L,Meola G

Fri Oct 22 00:00:00 EDT 2021

Tau-tubulin kinase 1 phosphorylates TDP-43 at disease-relevant sites and exacerbates TDP-43 pathology.

Neurobiology of disease

Tian Y,Wang Y,Jablonski AM,Hu Y,Sugam JA,Koglin M,Stachel SJ,Zhou H,Uslaner JM,Parmentier-Batteur S

Wed Dec 01 00:00:00 EST 2021

Icaritin protects SH-SY5Y cells transfected with TDP-43 by alleviating mitochondrial damage and oxidative stress.

PeerJ

Zhou Y,Huang N,Li Y,Ba Z,Zhou Y,Luo Y

Tue Apr 26 00:00:00 EDT 2022

Anatomic survey of seeding in Alzheimer's disease brains reveals unexpected patterns.

Acta neuropathologica communications

Stopschinski BE,Del Tredici K,Estill-Terpack SJ,Ghebremdehin E,Yu FF,Braak H,Diamond MI

Mon Oct 11 00:00:00 EDT 2021

TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.

Nature neuroscience

Laferrière F,Maniecka Z,Pérez-Berlanga M,Hruska-Plochan M,Gilhespy L,Hock EM,Wagner U,Afroz T,Boersema PJ,Barmettler G,Foti SC,Asi YT,Isaacs AM,Al-Amoudi A,Lewis A,Stahlberg H,Ravits J,De Giorgi F,Ichas F,Bezard E,Picotti P,Lashley T,Polymenidou M

Tue Jan 01 00:00:00 EST 2019

TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.

Nature neuroscience

Tue Jan 01 00:00:00 EST 2019

Pathological Correlates of Cognitive Impairment in The University of Manchester Longitudinal Study of Cognition in Normal Healthy Old Age.

Journal of Alzheimer's disease : JAD

Robinson AC,Davidson YS,Horan MA,Pendleton N,Mann DMA

Mon Jul 15 00:00:00 EDT 2019

Viral delivery of C9orf72 hexanucleotide repeat expansions in mice leads to repeat-length-dependent neuropathology and behavioural deficits.

Disease models & mechanisms

Herranz-Martin S,Chandran J,Lewis K,Mulcahy P,Higginbottom A,Walker C,Valenzuela IMY,Jones RA,Coldicott I,Iannitti T,Akaaboune M,El-Khamisy SF,Gillingwater TH,Shaw PJ,Azzouz M

Sat Jul 01 00:00:00 EDT 2017

Viral delivery of C9orf72 hexanucleotide repeat expansions in mice leads to repeat-length-dependent neuropathology and behavioural deficits.

Disease models & mechanisms

Herranz-Martin S,Chandran J,Lewis K,Mulcahy P,Higginbottom A,Walker C,Valenzuela IMY,Jones RA,Coldicott I,Iannitti T,Akaaboune M,El-Khamisy SF,Gillingwater TH,Shaw PJ,Azzouz M

Sat Jul 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43

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Gene Aliases: 1190002A23Rik; ALS10; C85084; TARDBP; TDP-43; TDP43

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UniProt ID: (Human) Q13148, (Mouse) Q921F2

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Entrez Gene ID: (Human) 23435, (Mouse) 230908, (Rat) 298648

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Function(s)

nucleotide binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA binding mRNA 3'-UTR binding protein binding identical protein binding poly(A) RNA binding nucleic acid binding DNA binding

Process(es)

negative regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mRNA processing RNA splicing negative regulation of gene expression nuclear fragmentation involved in apoptotic nuclear change negative regulation by host of viral transcription positive regulation of transcription from RNA polymerase II promoter regulation of cell cycle 3'-UTR-mediated mRNA stabilization nuclear inner membrane organization positive regulation of insulin secretion response to endoplasmic reticulum stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TDP-43 Polyclonal Antibody

TDP-43 Antibody (18280-1-AP) in ICC/IF

Product Details

18280-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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