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Invitrogen
TDP-43 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
TDP-43 Antibody (PA5-19712) in WB
Western blot was performed using Anti-TDP-43 Polyclonal Antibody(Product # PA5-19712) and a 44kDa band corresponding to TDP-43 was observed across cell lines tested. Whole Cell Extract-WCL (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Caco-2 (Lane 3), K-562 (Lane 4), Neuro-2a (Lane 5) and NIH/3T3 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blo... View More
Product Details
PA5-19712
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human TARDBP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10980717
Product Specific Information
PA5-19712 targets TARDBP in IF, IHC (F), IP, and WB applications and shows reactivity with Human and mouse samples.
The PA5-19712 immunogen is synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human TARDBP.
PA5-19712 detects TARDBP which has a predicted molecular weight of approximately 47 kDa.
Target Information
TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3"e; splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000002171; OTTHUMP00000002173; RP4-635E18.2; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TDP-43
Gene Aliases: ALS10; TARDBP; TDP-43; TDP43
UniProt ID: (Human) Q13148
Entrez Gene ID: (Human) 23435
negative regulation of protein phosphorylation
transcription from RNA polymerase II promoter
mRNA processing
RNA splicing
negative regulation of gene expression
nuclear fragmentation involved in apoptotic nuclear change
positive regulation of insulin secretion
response to endoplasmic reticulum stress
negative regulation by host of viral transcription
positive regulation of transcription from RNA polymerase II promoter
regulation of cell cycle
3'-UTR-mediated mRNA stabilization
nuclear inner membrane organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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