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TEB4 Antibody (PA5-103816) in ICC/IF
Immunocytochemistry analysis of TEB4 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with TEB4 polyclonal antibody (Product # PA5-103816) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103816
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MARCH6(Accession O60337), corresponding to amino acid residues Q866-E910.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853149
Product Specific Information
Antibody detects endogenous levels of total MARCH6.
Target Information
MARCH6 is an E3 ubiquitin ligase that localizes to endoplasmic reticulum membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 6-Mar; Doa10 homolog; E3 ubiquitin-protein ligase MARCH6; E3 ubiquitin-protein ligase MARCHF6; MARCH-VI; membrane associated ring finger 6; membrane-associated ring finger (C3HC4) 6, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 6; membrane-associated RING-CH finger protein 6; Membrane-associated RING-CH protein VI; Protein TEB-4; RING finger protein 176; RING-type E3 ubiquitin transferase MARCHF6
Gene Aliases: 3830408G03; DOA10; F830029L24Rik; KIAA0597; MARCH-VI; MARCH6; MARCHF6; mKIAA0597; RNF176; TEB4
UniProt ID: (Human) O60337, (Mouse) Q6ZQ89
Entrez Gene ID: (Human) 10299, (Mouse) 223455
ubiquitin-protein transferase activity
zinc ion binding
ligase activity
enzyme binding
ubiquitin conjugating enzyme binding
ubiquitin protein ligase activity involved in ERAD pathway
ubiquitin-specific protease binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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