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TFAM Antibody (PA5-27865) in ICC/IF
Immunofluorescence analysis of mtTFA was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with mtTFA Rabbit Polyclonal Antibody (Product # PA5-27865) at 1:500 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:200... View More
Product Details
PA5-27865
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 246 of Human mtTFA
Conjugate
Form
Liquid
Concentration
1.23 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545341
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2.
Predicted reactivity: Chimpanzee (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes, but it is transported from the cytoplasm to mitochondria where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates the replication and transcription of mtDNA, ensuring the proper functioning of mitochondrial processes. Structurally, TFAM contains a molecular region known as the LC3 interacting region (LIR) motif, which allows it to bind to autophagy proteins such as LC3. This interaction is essential for the autolysosomal pathway, helping to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, with notable effects on mitochondrial function; high levels can repress mtDNA expression in certain tissues, such as skeletal muscle, and contribute to deficiencies in oxidative phosphorylation (OXPHOS). Conversely, in other tissues like the heart, increased mtDNA copy number maintains a balanced TFAM-to-mtDNA ratio, preserving OXPHOS capacity. TFAM is integral to the packaging, stability, and replication of the mitochondrial genome, and disruptions in TFAM can have severe effects, including implications in neurodegeneration and other diseases related to mtDNA stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mitochondrial transcription factor 1; mitochondrial transcription factor A; MtTF1; mtTFA; TCF-6; Testis-specific high mobility group protein; testis-specific HMG-box protein m-tsHMG; Transcription factor 6; transcription factor 6-like 1; Transcription factor 6-like 2; transcription factor 6-like 2 (mitochondrial transcription factor); transcription factor 6-like 3; Transcription factor A, mitochondrial; TS-HMG
Gene Aliases: AI661103; Hmgts; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; tsHMG
UniProt ID: (Human) Q00059, (Rat) Q91ZW1, (Mouse) P40630
Entrez Gene ID: (Human) 7019, (Rat) 83474, (Mouse) 21780
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
DNA binding, bending
heat shock protein binding
poly(A) RNA binding
mitochondrial light strand promoter sense binding
DNA binding
mitochondrial DNA replication
chromatin remodeling
regulation of transcription, DNA-templated
transcription from mitochondrial promoter
transcription initiation from mitochondrial promoter
mitochondrial respiratory chain complex assembly
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
DNA-dependent DNA replication
regulation of transcription from RNA polymerase I promoter
transcription from RNA polymerase II promoter
mitochondrion organization
transcription, DNA-templated
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