Proteintech

TGFBI / BIGH3 Monoclonal Antibody (3E11D11)

View all (24) TGFBI antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (9)

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

TGFBI / BIGH3 Antibody (60007-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human colon tissue slide using (Product # 60007-1-Ig) TGFBI/BIGH3 antibody at dilution of 1:1,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TGFBI / BIGH3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

TGFBI / BIGH3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

TGFBI / BIGH3 Antibody in Western Blot (WB)

TGFBI / BIGH3 Antibody in Western Blot (WB)

TGFBI / BIGH3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human colon tissue slide using (Product # 60007-1-Ig) TGFBI/BIGH3 antibody at dilution of 1:1,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human colon tissue slide using (Product # 60007-1-Ig) TGFBI/BIGH3 antibody at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 60007-1-IG (TGFBI / BIGH3 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TGFBI / BIGH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 60007-1-IG (TGFBI / BIGH3 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TGFBI / BIGH3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TGFBI / BIGH3 antibody (Product # 60007-1-IG, Clone: 3E11D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

TGFBI / BIGH3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed Human colon cancer tissue using TGFBI/BIGH3 antibody (Product # 60007-1-Ig) (Clone: 3E11D11) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

TGFBI / BIGH3 Antibody in Western Blot (WB) — Human kidney tissue were subjected to SDS PAGE followed by western blot with (Product # 60007-1-Ig) TGFBI/BIGH3 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

TGFBI / BIGH3 Antibody in Western Blot (WB) — WB detection of anti-TGFBI in the extracellularmedia of LEC grown for 24 h under normoxia (N), hypoxia (H) or hypoxia and reoxygenation (H/N) for 6 h. One result representative of at least three different experiments is shown. Silver staining of the membranes was used as loading control. (From PMID:18560760; Irigoyen M, et al, TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions, Cell Mol Life Sci. 65 (14), 2244-55, 2008).

TGFBI / BIGH3 Antibody in Immunoprecipitation (IP) — IP result of anti-TGFBI / BIGH3 (IP:60007-1-IG, 4ug; Detection:60007-1-IG 1:300) with HeLa cells lysate 1200ug.

Product Details

60007-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3E11D11

Immunogen

TGFBI / BIGH3 Fusion Protein Ag0241 (199-406 aa encoded by BC000097)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Caprylic acid precipitation

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: NIQIHHYPN GIVTVNCARL LKADHHATNG VVHLIDKVIS TITNNIQQII EIEDTFETLR AAVAASGLNT MLEGNGQYTL LAPTNEAFEK IPSETLNRIL GDPEALRDLL NNHILKSAMC AEAIVAGLSV ETLEGTTLEV GCSGDMLTIN GKAIISNKDI LATNGVIHYI DELLIPDSAK TLFELAAESD VSTAIDLFRQ AGLGNHLSG (199-406 aa encoded by BC000097)

Target Information

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis.

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Li M,Wang X,Yang E,Li Y,Geng Y,Chen Z,Huang S,Zhang D,Wu H

Mon May 01 00:00:00 EDT 2023

OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis.

Arteriosclerosis, thrombosis, and vascular biology

Li M,Wang X,Yang E,Li Y,Geng Y,Chen Z,Huang S,Zhang D,Wu H

Mon May 01 00:00:00 EDT 2023

OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis.

Arteriosclerosis, thrombosis, and vascular biology

Li M,Wang X,Yang E,Li Y,Geng Y,Chen Z,Huang S,Zhang D,Wu H

Mon May 01 00:00:00 EDT 2023

OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis.

Arteriosclerosis, thrombosis, and vascular biology

Li M,Wang X,Yang E,Li Y,Geng Y,Chen Z,Huang S,Zhang D,Wu H

Mon May 01 00:00:00 EDT 2023

OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis.

Arteriosclerosis, thrombosis, and vascular biology

Li M,Wang X,Yang E,Li Y,Geng Y,Chen Z,Huang S,Zhang D,Wu H

Mon May 01 00:00:00 EDT 2023

OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis.

Arteriosclerosis, thrombosis, and vascular biology

Li M,Wang X,Yang E,Li Y,Geng Y,Chen Z,Huang S,Zhang D,Wu H

Mon May 01 00:00:00 EDT 2023

OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis.

Arteriosclerosis, thrombosis, and vascular biology

Li M,Wang X,Yang E,Li Y,Geng Y,Chen Z,Huang S,Zhang D,Wu H

Mon May 01 00:00:00 EDT 2023

OTUB1 Catalytic-Independently Deubiquitinates TGFBI and Mediates the Angiogenesis in Infantile Hemangioma by Regulating Glycolysis.

Arteriosclerosis, thrombosis, and vascular biology

Li M,Wang X,Yang E,Li Y,Geng Y,Chen Z,Huang S,Zhang D,Wu H

Mon May 01 00:00:00 EDT 2023

TGFBI Production by Macrophages Contributes to an Immunosuppressive Microenvironment in Ovarian Cancer.

Cancer research

Lecker LSM,Berlato C,Maniati E,Delaine-Smith R,Pearce OMT,Heath O,Nichols SJ,Trevisan C,Novak M,McDermott J,Brenton JD,Cutillas PR,Rajeeve V,Hennino A,Drapkin R,Loessner D,Balkwill FR

Mon Nov 15 00:00:00 EST 2021

TGFBI Production by Macrophages Contributes to an Immunosuppressive Microenvironment in Ovarian Cancer.

Cancer research

Lecker LSM,Berlato C,Maniati E,Delaine-Smith R,Pearce OMT,Heath O,Nichols SJ,Trevisan C,Novak M,McDermott J,Brenton JD,Cutillas PR,Rajeeve V,Hennino A,Drapkin R,Loessner D,Balkwill FR

Mon Nov 15 00:00:00 EST 2021

TGFBI Production by Macrophages Contributes to an Immunosuppressive Microenvironment in Ovarian Cancer.

Cancer research

Lecker LSM,Berlato C,Maniati E,Delaine-Smith R,Pearce OMT,Heath O,Nichols SJ,Trevisan C,Novak M,McDermott J,Brenton JD,Cutillas PR,Rajeeve V,Hennino A,Drapkin R,Loessner D,Balkwill FR

Mon Nov 15 00:00:00 EST 2021

TGFBI Production by Macrophages Contributes to an Immunosuppressive Microenvironment in Ovarian Cancer.

Cancer research

Lecker LSM,Berlato C,Maniati E,Delaine-Smith R,Pearce OMT,Heath O,Nichols SJ,Trevisan C,Novak M,McDermott J,Brenton JD,Cutillas PR,Rajeeve V,Hennino A,Drapkin R,Loessner D,Balkwill FR

Mon Nov 15 00:00:00 EST 2021

Identification of Hub Genes Associated With Sensitivity of 5-Fluorouracil Based Chemotherapy for Colorectal Cancer by Integrated Bioinformatics Analysis.

Frontiers in oncology

Wang Y,Wei Q,Chen Y,Long S,Yao Y,Fu K

Fri Apr 30 00:00:00 EDT 2021

BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.

Neoplasia (New York, N.Y.)

Pan T,Lin SC,Yu KJ,Yu G,Song JH,Lewis VO,Bird JE,Moon B,Lin PP,Tannir NM,Jonasch E,Wood CG,Gallick GE,Yu-Lee LY,Lin SH,Satcher RL

Mon Jan 01 00:00:00 EST 2018

BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.

Neoplasia (New York, N.Y.)

Pan T,Lin SC,Yu KJ,Yu G,Song JH,Lewis VO,Bird JE,Moon B,Lin PP,Tannir NM,Jonasch E,Wood CG,Gallick GE,Yu-Lee LY,Lin SH,Satcher RL

Mon Jan 01 00:00:00 EST 2018

BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.

Neoplasia (New York, N.Y.)

Pan T,Lin SC,Yu KJ,Yu G,Song JH,Lewis VO,Bird JE,Moon B,Lin PP,Tannir NM,Jonasch E,Wood CG,Gallick GE,Yu-Lee LY,Lin SH,Satcher RL

Mon Jan 01 00:00:00 EST 2018

BIGH3 Promotes Osteolytic Lesions in Renal Cell Carcinoma Bone Metastasis by Inhibiting Osteoblast Differentiation.

Neoplasia (New York, N.Y.)

Pan T,Lin SC,Yu KJ,Yu G,Song JH,Lewis VO,Bird JE,Moon B,Lin PP,Tannir NM,Jonasch E,Wood CG,Gallick GE,Yu-Lee LY,Lin SH,Satcher RL

Mon Jan 01 00:00:00 EST 2018

Absence of Vitamin K-Dependent γ-Carboxylation in Human Periostin Extracted from Fibrotic Lung or Secreted from a Cell Line Engineered to Optimize γ-Carboxylation.

PloS one

Annis DS,Ma H,Balas DM,Kumfer KT,Sandbo N,Potts GK,Coon JJ,Mosher DF

Fri May 06 00:00:00 EDT 2016

Absence of Vitamin K-Dependent γ-Carboxylation in Human Periostin Extracted from Fibrotic Lung or Secreted from a Cell Line Engineered to Optimize γ-Carboxylation.

PloS one

Annis DS,Ma H,Balas DM,Kumfer KT,Sandbo N,Potts GK,Coon JJ,Mosher DF

Fri May 06 00:00:00 EDT 2016

Transforming growth factor-beta-induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells.

International journal of cancer

Ween MP,Lokman NA,Hoffmann P,Rodgers RJ,Ricciardelli C,Oehler MK

Fri Apr 01 00:00:00 EDT 2011

Transforming growth factor-beta-induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells.

International journal of cancer

Ween MP,Lokman NA,Hoffmann P,Rodgers RJ,Ricciardelli C,Oehler MK

Fri Apr 01 00:00:00 EDT 2011

Transforming growth factor-beta-induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells.

International journal of cancer

Ween MP,Lokman NA,Hoffmann P,Rodgers RJ,Ricciardelli C,Oehler MK

Fri Apr 01 00:00:00 EDT 2011

Transforming growth factor-beta-induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells.

International journal of cancer

Ween MP,Lokman NA,Hoffmann P,Rodgers RJ,Ricciardelli C,Oehler MK

Fri Apr 01 00:00:00 EDT 2011

TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions.

Cellular and molecular life sciences : CMLS

Irigoyen M,Ansó E,Salvo E,Dotor de las Herrerías J,Martínez-Irujo JJ,Rouzaut A

Tue Jul 01 00:00:00 EDT 2008

TGFbeta-induced protein mediates lymphatic endothelial cell adhesion to the extracellular matrix under low oxygen conditions.

Cellular and molecular life sciences : CMLS

Irigoyen M,Ansó E,Salvo E,Dotor de las Herrerías J,Martínez-Irujo JJ,Rouzaut A

Tue Jul 01 00:00:00 EDT 2008

Modified BIGH3 with an RGDRGD motif promotes human corneal epithelial cell adhesion and migration in vitro.

Current eye research

Ge H,Cao W,Leng F,Chen W,Liu P,Chen W

Sat Mar 01 00:00:00 EST 2008

Modified BIGH3 with an RGDRGD motif promotes human corneal epithelial cell adhesion and migration in vitro.

Current eye research

Ge H,Cao W,Leng F,Chen W,Liu P,Chen W

Sat Mar 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: Beta ig-h3; beta-ig-h3; betaig-h3; Kerato-epithelin; RGD-CAP; RGD-containing collagen-associated protein; transforming growth factor beta-induced 68kDa; transforming growth factor, beta-induced, 68kD; transforming growth factor, beta-induced, 68kDa; Transforming growth factor-beta-induced protein ig-h3

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Gene Aliases: BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1; TGFBI

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UniProt ID: (Human) Q15582

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Entrez Gene ID: (Human) 7045

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integrin binding protein binding collagen binding extracellular matrix binding cell adhesion molecule

Process(es)

angiogenesis chondrocyte differentiation cell adhesion negative regulation of cell adhesion visual perception cell proliferation extracellular matrix organization cellular protein metabolic process response to stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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