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FIGURE: 1 / 4
TGN38 Antibody (OST00228G-500UG) in ICC/IF
Human Melanoma cell line C32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 µl of Rabbit antibody to TGN (OST00228G) diluted 1:100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 µl of anti Rb-FITC... View More
Product Details
OST00228G-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat,
Human,
Mouse,
Non-human primate
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from mouse TGN38 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: TGN38 (or TGN46, TGN48, TGN51 in human).
Target Information
IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1. and ASPP2. are activators of p53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hTGN48; hTGN51; TGN38 homolog; TGN38B; Trans-Golgi network glycoprotein 46; Trans-Golgi network glycoprotein 48; Trans-Golgi network glycoprotein 51; Trans-Golgi network integral membrane protein 2; Trans-Golgi network integral membrane protein TGN38; trans-Golgi network protein (46, 48, 51kD isoforms); trans-golgi network protein 1; Trans-Golgi network protein 2; trans-Golgi network protein TGN51
Gene Aliases: TGN38; TGN38B; TGN41; TGN46; TGN48; TGN51; Tgoln1; TGOLN2; Ttgn1; TTGN2
UniProt ID: (Human) O43493, (Rat) P19814, (Mouse) Q62314
Entrez Gene ID: (Human) 10618, (Rat) 192152, (Mouse) 22135
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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