Invitrogen

TGN46 Polyclonal Antibody

TGN46 Antibody in Immunocytochemistry (ICC/IF)

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TGN46 Antibody (PA5-23068) in ICC/IF

Immunofluorescence analysis of TGN46 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TGN46 Polyclonal Antibody (Product # PA5-23068) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

TGN46 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-23068

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunocytochemistry (ICC/IF)

1:500

View 7 publications 7 publications

Flow Cytometry (Flow)

Assay-Dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Non-human primate

Published species

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an internal region of the human TGOLN2 protein (within residues 200-350).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11156003

Product Specific Information

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Target Information

IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1. and ASPP2. are activators of p53.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Diffusion Model of Intra-Golgi Transport Has Limited Power.

International journal of molecular sciences

Beznoussenko GV,Bejan AI,Parashuraman S,Luini A,Kweon HS,Mironov AA

PA5-23068 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to validate the goodness-of-fit of the regression lines describing the exit of a cargo from the Golgi zone correspond to an exponential decay.

Tue Jan 10 00:00:00 EST 2023

Virus-Free Method to Control and Enhance Extracellular Vesicle Cargo Loading and Delivery.

ACS applied bio materials

Bui S,Dancourt J,Lavieu G

PA5-23068 was used in Western Blotting to develop two synergistic methods to, respectively, control EV cargo loading and enhance EV cargo delivery through fusion without requirement for any viral fusogenic protein.

Mon Mar 20 00:00:00 EDT 2023

Nitric Oxide Inhibition of Rickettsia rickettsii.

Infection and immunity

Fitzsimmons LF,Clark TR,Hackstadt T

PA5-23068 was used in Immunocytochemistry-immunoflourescence to indicate that NO is a potent antirickettsial effector of innate immunity that targets energy generation in these pathogenic bacteria to prevent growth and subversion of infected host cells.

Tue Nov 16 00:00:00 EST 2021

PML nuclear body-residing proteins sequentially associate with HPV genome after infectious nuclear delivery.

PLoS pathogens

Guion L,Bienkowska-Haba M,DiGiuseppe S,Florin L,Sapp M

PA5-23068 was used in Immunocytochemistry-immunoflourescence to test the hypothesis that PML protein is recruited to incoming viral genome prior to egress from transport vesicles.

Fri Feb 01 00:00:00 EST 2019

The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.

Developmental cell

Procter DJ,Banerjee A,Nukui M,Kruse K,Gaponenko V,Murphy EA,Komarova Y,Walsh D

PA5-23068 was used in Immunocytochemistry to show that the assembly compartment also acts as a microtubule-organizing center (MTOC) wherein centrosome activity is suppressed and Golgi-based microtubule (MT) nucleation is enhanced.

Mon Apr 09 00:00:00 EDT 2018

Human Papillomavirus Major Capsid Protein L1 Remains Associated with the Incoming Viral Genome throughout the Entry Process.

Journal of virology

DiGiuseppe S,Bienkowska-Haba M,Guion LGM,Keiffer TR,Sapp M

PA5-23068 was used in Immunocytochemistry to demonstrate that human papillomavirus conformationally intact L1 protein, likely still arranged as capsomeres, is associated with the viral genome throughout mitosis and transiently resides in the nucleus until after the viral DNA is released from the transport vesicle.

Tue Aug 15 00:00:00 EDT 2017

Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection.

Open biology

Pavelin J,McCormick D,Chiweshe S,Ramachandran S,Lin YT,Grey F

Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection.

Open biology

Pavelin J,McCormick D,Chiweshe S,Ramachandran S,Lin YT,Grey F

Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Incoming human papillomavirus type 16 genome resides in a vesicular compartment throughout mitosis.

Proceedings of the National Academy of Sciences of the United States of America

DiGiuseppe S,Luszczek W,Keiffer TR,Bienkowska-Haba M,Guion LG,Sapp MJ

Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunofluorescence

Tue May 31 00:00:00 EDT 2016

Topography of the Human Papillomavirus Minor Capsid Protein L2 during Vesicular Trafficking of Infectious Entry.

Journal of virology

DiGiuseppe S,Keiffer TR,Bienkowska-Haba M,Luszczek W,Guion LG,Müller M,Sapp M

PA5-23068 was used in immunocytochemistry to map the topography of the human papillomavirus L2 protein during infection

Thu Oct 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: hTGN48; hTGN51; TGN38 homolog; TGN38B; Trans-Golgi network glycoprotein 46; Trans-Golgi network glycoprotein 48; Trans-Golgi network glycoprotein 51; Trans-Golgi network integral membrane protein 2; trans-Golgi network protein (46, 48, 51kD isoforms); Trans-Golgi network protein 2; trans-Golgi network protein TGN51

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Gene Aliases: TGN38; TGN38B; TGN41; TGN46; TGN48; TGN51; TGOLN2; TTGN2

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UniProt ID: (Human) O43493, (Mouse) Q62314

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Entrez Gene ID: (Human) 10618, (Mouse) 22135

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Function(s)

protein binding membrane traffic protein

Process(es)

Golgi to endosome transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TGN46 Polyclonal Antibody

TGN46 Antibody (PA5-23068) in ICC/IF

Product Details

PA5-23068

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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