Proteintech

TH Monoclonal Antibody (2H7B7)

TH Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

TH Antibody (66334-1-IG) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 66334-1-IG (TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

TH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

66334-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2H7B7

Immunogen

TH Fusion Protein Ag23075 (1-260 aa encoded by BC104967)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPTPDATTP QAKGFRRAVS ELDAKQAEAI MSPRFIGRRQ SLIEDARKER EAAVAAAAAA VPSEPGDPLE AVAFEEKEGK AMLNLLFSPR ATKPSALSRA VKVFETFEAK IHHLETRPAQ RPRAGGPHLE YFVRLEVRRG DLAALLSGVR QVSEDVRSPA GPKVPWFPRK VSELDKCHHL VTKFDPDLDL DHPGFSDQVY RQRRKLIAEI AFQYRHGDPI PRVEYTAEEI ATWKEVYTTL KGLYATHACG EHLEAFALLE R (1-260 aa encoded by BC104967)

Target Information

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activation of HCA2 regulates microglial responses to alleviate neurodegeneration in LPS-induced in vivo and in vitro models.

Journal of neuroinflammation

He D,Fu S,Ye B,Wang H,He Y,Li Z,Li J,Gao X,Liu D

Wed Mar 29 00:00:00 EDT 2023

microRNAs profiling of small extracellular vesicles from midbrain tissue of Parkinson's disease.

Frontiers in molecular neuroscience

Li Z,Chen D,Pan R,Zhong Y,Zhong T,Jiao Z

Fri Feb 24 00:00:00 EST 2023

Effect of High-Intensity Interval Training on Fatty Infiltration After Delayed Rotator Cuff Repair in a Mouse Model.

Orthopaedic journal of sports medicine

Zhou H,Wang Z,Chen C,Hu H,Jiang B,Yin Y,Zhang K,Shen M,Wu S

Mon May 01 00:00:00 EDT 2023

Effect of High-Intensity Interval Training on Fatty Infiltration After Delayed Rotator Cuff Repair in a Mouse Model.

Orthopaedic journal of sports medicine

Zhou H,Wang Z,Chen C,Hu H,Jiang B,Yin Y,Zhang K,Shen M,Wu S

Mon May 01 00:00:00 EDT 2023

Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency.

Antioxidants (Basel, Switzerland)

Sun X,Dong S,Kato H,Kong J,Ito Y,Hirofuji Y,Sato H,Kato TA,Sakai Y,Ohga S,Fukumoto S,Masuda K

Wed Jul 13 00:00:00 EDT 2022

Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Bian LH,Yao ZW,Wang ZY,Wang XM,Li QY,Yang X,Li JY,Wei XJ,Wan GH,Wang YQ,Shi JL,Guo JY

Thu Sep 01 00:00:00 EDT 2022

FASEB bioAdvances

Sun X,Kato H,Sato H,Han X,Hirofuji Y,Kato TA,Sakai Y,Ohga S,Fukumoto S,Masuda K

Fri Jul 01 00:00:00 EDT 2022

FASEB bioAdvances

Sun X,Kato H,Sato H,Han X,Hirofuji Y,Kato TA,Sakai Y,Ohga S,Fukumoto S,Masuda K

Fri Jul 01 00:00:00 EDT 2022

Pro-metastatic and mesenchymal gene expression signatures characterize circulating tumor cells of neuroblastoma patients with bone marrow metastases and relapse.

Frontiers in oncology

Loh AHP,Angelina C,Wong MK,Tan SH,Sukhatme SA,Yeo T,Lim SB,Lee YT,Soh SY,Leung W,Chang KTE,Chua YW,Alkaff SMF,Lim TKH,Lim CT,Chen ZX

Sat Oct 01 00:00:00 EDT 2022

A novel method for generating glutamatergic SH-SY5Y neuron-like cells utilizing B-27 supplement.

Frontiers in pharmacology

Martin ER,Gandawijaya J,Oguro-Ando A

Tue Nov 08 00:00:00 EST 2022

Transplantation of Nurr1-overexpressing neural stem cells and microglia for treating parkinsonian rats.

CNS neuroscience & therapeutics

Qian Y,Chen XX,Wang W,Li JJ,Wang XP,Tang ZW,Xu JT,Lin H,Yang ZY,Li LY,Song XB,Guo JZ,Bian LG,Zhou L,Lu D,Deng XL

Wed Jan 01 00:00:00 EST 2020

RAB39B Deficiency Impairs Learning and Memory Partially Through Compromising Autophagy.

Frontiers in cell and developmental biology

Niu M,Zheng N,Wang Z,Gao Y,Luo X,Chen Z,Fu X,Wang Y,Wang T,Liu M,Yao T,Yao P,Meng J,Zhou Y,Ge Y,Wang Z,Ma Q,Xu H,Zhang YW

Tue Dec 29 00:00:00 EST 2020

Norepinephrine-CREB1-miR-373 axis promotes progression of colon cancer.

Molecular oncology

Han J,Jiang Q,Ma R,Zhang H,Tong D,Tang K,Wang X,Ni L,Miao J,Duan B,Yang Y,Chen Y,Wu F,Han J,Wang M,Hou N,Huang C

Fri May 01 00:00:00 EDT 2020

Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.

Journal of neuropathology and experimental neurology

Liu W,Fu R,Wang Z,Liu S,Tang C,Li L,Yin D

Wed Jan 01 00:00:00 EST 2020

Asparagine endopeptidase inhibitor protects against fenpropathrin-induced neurodegeneration via suppressing α-synuclein aggregation and neuroinflammation.

European journal of pharmacology

Yu T,Wan F,Liu C,Zhang X,Liu Z,Zhang J,Xiong J,Wang T,Zhang Z

Sat Dec 05 00:00:00 EST 2020

Wnt1 silencing enhances neurotoxicity induced by paraquat and maneb in SH-SY5Y cells.

Experimental and therapeutic medicine

Huang C,Ma J,Li BX,Sun Y

Fri Nov 01 00:00:00 EDT 2019

Hydralazine Protects Nigrostriatal Dopaminergic Neurons From MPP⁺ and MPTP Induced Neurotoxicity: Roles of Nrf2-ARE Signaling Pathway.

Frontiers in neurology

Guo X,Han C,Ma K,Xia Y,Wan F,Yin S,Kou L,Sun Y,Wu J,Hu J,Huang J,Xiong N,Wang T

Wed Sep 30 00:00:00 EDT 2020

Mitochondrial dysfunction in dopaminergic neurons differentiated from exfoliated deciduous tooth-derived pulp stem cells of a child with Rett syndrome.

Biochemical and biophysical research communications

Hirofuji S,Hirofuji Y,Kato H,Masuda K,Yamaza H,Sato H,Takayama F,Torio M,Sakai Y,Ohga S,Taguchi T,Nonaka K

Sun Apr 15 00:00:00 EDT 2018

Mitochondrial dysfunction in dopaminergic neurons differentiated from exfoliated deciduous tooth-derived pulp stem cells of a child with Rett syndrome.

Biochemical and biophysical research communications

Hirofuji S,Hirofuji Y,Kato H,Masuda K,Yamaza H,Sato H,Takayama F,Torio M,Sakai Y,Ohga S,Taguchi T,Nonaka K

Sun Apr 15 00:00:00 EDT 2018

Inhibition of HDAC6 increases acetylation of peroxiredoxin1/2 and ameliorates 6-OHDA induced dopaminergic injury.

Neuroscience letters

Jian W,Wei X,Chen L,Wang Z,Sun Y,Zhu S,Lou H,Yan S,Li X,Zhou J,Zhang B

Fri Sep 29 00:00:00 EDT 2017

Inhibition of HDAC6 increases acetylation of peroxiredoxin1/2 and ameliorates 6-OHDA induced dopaminergic injury.

Neuroscience letters

Jian W,Wei X,Chen L,Wang Z,Sun Y,Zhu S,Lou H,Yan S,Li X,Zhou J,Zhang B

Fri Sep 29 00:00:00 EDT 2017

Inhibition of HDAC6 increases acetylation of peroxiredoxin1/2 and ameliorates 6-OHDA induced dopaminergic injury.

Neuroscience letters

Jian W,Wei X,Chen L,Wang Z,Sun Y,Zhu S,Lou H,Yan S,Li X,Zhou J,Zhang B

Fri Sep 29 00:00:00 EDT 2017

Inhibition of HDAC6 increases acetylation of peroxiredoxin1/2 and ameliorates 6-OHDA induced dopaminergic injury.

Neuroscience letters

Jian W,Wei X,Chen L,Wang Z,Sun Y,Zhu S,Lou H,Yan S,Li X,Zhou J,Zhang B

Fri Sep 29 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

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Gene Aliases: DYT14; DYT5b; TH; TYH

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UniProt ID: (Human) P07101

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Entrez Gene ID: (Human) 7054

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Function(s)

tyrosine 3-monooxygenase activity protein binding ferrous iron binding ferric iron binding amino acid binding oxygen binding enzyme binding protein domain specific binding tetrahydrobiopterin binding dopamine binding

Process(es)

response to hypoxia synaptic transmission, dopaminergic response to amphetamine heart morphogenesis dopamine biosynthetic process from tyrosine fatty acid metabolic process sphingolipid metabolic process heart development visual perception sensory perception of sound learning memory mating behavior locomotory behavior regulation of heart contraction response to water deprivation response to light stimulus response to herbicide response to salt stress anatomical structure morphogenesis organ morphogenesis response to zinc ion multicellular organism aging response to activity aminergic neurotransmitter loading into synaptic vesicle glycoside metabolic process phthalate metabolic process cerebral cortex development response to nutrient levels response to estradiol response to lipopolysaccharide isoquinoline alkaloid metabolic process social behavior cellular response to drug response to isolation stress response to immobilization stress neurotransmitter biosynthetic process terpene metabolic process dopamine biosynthetic process epinephrine biosynthetic process norepinephrine biosynthetic process catecholamine biosynthetic process eye photoreceptor cell development circadian sleep/wake cycle eating behavior response to peptide hormone pigmentation response to ethanol response to ether response to pyrethroid embryonic camera-type eye morphogenesis response to corticosterone response to electrical stimulus phytoalexin metabolic process oxidation-reduction process cellular response to manganese ion cellular response to nicotine cellular response to glucose stimulus cellular response to growth factor stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TH Monoclonal Antibody (2H7B7)

TH Antibody (66334-1-IG) in ICC/IF

Product Details

66334-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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