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THRB Antibody (PA1-213A) in ICC/IF
Immunofluorescent analysis of Thyroid Hormone Receptor beta-1 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thyroid Hormone Receptor beta-1 polyclonal antibody (Product # PA1-213A) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. C... View More
Product Details
PA1-213A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Mouse,
Rabbit
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C-I(62) F H L D H D D V N D Q S V S S A Q T F(81) of human TR beta-1, with an N-terminal added cysteine.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2203030
Product Specific Information
PA1-213A detects thyroid hormone receptor (TR) beta-1 from human and rat tissues. This antibody does not detect TR alpha-1 or TRv alpha-2.
PA1-213A has been successfully used in Western blot, immunohistochemistry (paraffin), IF and gel shift procedures. By Western blot, this antibody detects a ~55 kDa protein representing recombinant human TRv beta-1 expressed in E. coli.
PA1-213A immunizing peptide corresponds to residues 62-81 of human TR beta-1, with an N-terminal added cysteine. Alternative numbering of this protein has this peptide corresponding to residues 67-86 of human TR beta-1. This sequence is completely conserved between human and rat TR beta-1.
Target Information
Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA-BETA; MGC126109; MGC126110; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta; thyroid hormone receptor beta 3; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR beta 1; TR-B1C-ERBA-2; TRbeta2Delta
Gene Aliases: C-ERBA-2; C-ERBA-BETA; ERBA2; GRTH; NR1A2; PRTH; RATT3REC; T3rec; THR1; THRB; THRB1; THRB2; TRbeta
UniProt ID: (Human) P10828
Entrez Gene ID: (Human) 7068, (Rat) 24831
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription corepressor activity
thyroid hormone receptor activity
protein binding
zinc ion binding
enzyme binding
chromatin DNA binding
sequence-specific DNA binding
thyroid hormone binding
double-stranded DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein homodimerization activity
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
sensory perception of sound
negative regulation of female receptivity
regulation of heart contraction
female courtship behavior
organ morphogenesis
intracellular receptor signaling pathway
steroid hormone mediated signaling pathway
positive regulation of transcription from RNA polymerase II promoter
Type I pneumocyte differentiation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
positive regulation of cell proliferation
regulation of lipid metabolic process
positive regulation of chondrocyte differentiation
positive regulation of ossification
negative regulation of transcription, DNA-templated
embryonic organ development
regulation of cholesterol metabolic process
regulation of triglyceride metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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