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Invitrogen
TIMD2 Chimeric Recombinant Rabbit Monoclonal Antibody (RMT2-14)
FIGURE: 1 / 1
TIMD2 Chimeric Antibody (MA5-48167) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of TIMD2 in mouse splenocytes using TIMD2 Chimeric Monoclonal Antibody (Product # MA5-48167). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with TIMD2 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from ... View More
Product Details
MA5-48167
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, lambda
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RMT2-14
Immunogen
This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074610
Product Specific Information
Specificity: This antibody is specific for murine TIM-2, a transmembrane protein that is expressed on activated T cells and preferentially on differentiated Th2 cells. A blockade of TIM-2/TIM-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. TIM-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.
Target Information
TIM-2 recognizes T-cell immunoglobulin mucin 2, a transmembrane glycoprotein and member of the immunoglobulin superfamily, expressed by activated lymphocytes, developmentally in the 8-cell embryo and blastocyst, and preferentially up-regulated in Th2 cells and down-regulated in Th1 cells. Tim-2 acts as a negative regulator of Th2 cells, and is implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-2 also acts as a receptor for Semaphorin 4A, a transmembrane protein expressed on dendritic cells and B cells, which plays a role in the guidance of axonal migration during neuronal development, and has been shown to enhance T cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: T-cell immunoglobulin and mucin domain containing 2; T-cell immunoglobulin and mucin domain-containing protein 2; T-cell immunoglobulin mucin receptor 2; T-cell membrane protein 2; TIM-2; TIMD-2
Gene Aliases: AA816106; AU019358; AU019662; C78111; G9D3; TIM-2; Tim2; Timd2
UniProt ID: (Mouse) Q8R183
Entrez Gene ID: (Mouse) 171284
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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