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TIMP1 Antibody (500-P280BT-25UG) in WB
Western Blot: To detect hTIMP-1 by Western Blot analysis TIMP1 Polyclonal Antibody, Biotin (Product # 500-P280BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTIMP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P280BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 20.6 kDa Recombinant Human TIMP-1
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930231
Product Specific Information
AA Sequence of recombinant protein: CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TIMP-1. Anti-Human TIMP-1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hTIMP-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TIMP-1 (500-P280) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTIMP-1.
Western Blot: To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTIMP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P280BT-1MG will be provided as 2 x 500 µg
Target Information
TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collagenase inhibitor; EPA; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; Metalloproteinase inhibitor; Metalloproteinase inhibitor 1; MMP inhibitor; RP1-230G1.3; TIMP 1; TIMP-1; Tissue inhibitor of metalloproteinases 1
Gene Aliases: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1
UniProt ID: (Human) P01033
Entrez Gene ID: (Human) 7076
cell activation
platelet degranulation
aging
positive regulation of cell proliferation
response to hormone
negative regulation of endopeptidase activity
extracellular matrix disassembly
response to cytokine
wound healing
negative regulation of apoptotic process
negative regulation of catalytic activity
response to peptide hormone
negative regulation of membrane protein ectodomain proteolysis
cartilage development
negative regulation of trophoblast cell migration
regulation of integrin-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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