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TIMP1 Antibody (PA5-47778) in WB
Western blot was performed using Anti-TIMP1 Polyclonal Antibody (Product # PA5-47778) and a 28 kDa band corresponding to TIMP1 was observed in MDA-MB-231 treated with protein transport inhibitor in comparison to Daudi treated with protein transport inhibitor which is reported to be negative. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with PTI for 4 hr (Lane 2), Daudi (Lane 3), Daudi treated with PTI for 4 hr (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Reso... View More
Product Details
PA5-47778
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TIMP-1 Cys24-Ala217
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609009
Product Specific Information
In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) TIMP-1 and recombinant mouse TIMP-1 is observed and less than 1% cross-reactivity with rhTIMP-2, rhTIMP-3, and rhTIMP-4 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collagenase inhibitor; EPA; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; Metalloproteinase inhibitor; Metalloproteinase inhibitor 1; MMP inhibitor; RP1-230G1.3; TIMP 1; TIMP-1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase 1; Tissue inhibitor of metalloproteinases 1
Gene Aliases: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1
UniProt ID: (Human) P01033, (Rat) P30120
Entrez Gene ID: (Human) 7076, (Rat) 116510
cell activation
platelet degranulation
aging
positive regulation of cell proliferation
response to hormone
negative regulation of endopeptidase activity
extracellular matrix disassembly
response to cytokine
wound healing
negative regulation of apoptotic process
negative regulation of catalytic activity
response to peptide hormone
negative regulation of membrane protein ectodomain proteolysis
cartilage development
negative regulation of trophoblast cell migration
regulation of integrin-mediated signaling pathway
steroid biosynthetic process
response to organic substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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