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TIMP3 Antibody (710404) in ICC/IF
Immunofluorescent analysis of TIMP3 in HeLa cells using a TIMP3 Recombinant Rabbit Polyclonal Antibody (Product # 710404) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of TIMP3.
Product Details
710404
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
20HCLC
Immunogen
proprietary
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532709
Product Specific Information
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
Tissue inhibitor of metalloproteinase 3 (TIMP3) is a member of the tissue inhibitor of metalloproteinases gene family. It functions to inhibit the activity of matrix metalloproteinases (MMP) which degrade components of the extracellular matrix. TIMP3 is expressed upon mitogenic stimulation. It binds irreversibly to zinc-dependent MMPs and inactivates them by binding to their catalytic zinc cofactor. Other functions of TIMP3 include nervous system development, tissue regeneration, and visual perception. In humans, the gene encoding TIMP3 is located on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; TIMP 3; TIMP-3; Tissue inhibitor of metalloproteinases 3
Gene Aliases: HSMRK222; K222; K222TA2; SFD; Sun; Timp-3; TIMP3
UniProt ID: (Human) P35625, (Mouse) P39876
Entrez Gene ID: (Human) 7078, (Mouse) 21859
platelet degranulation
central nervous system development
aging
visual perception
response to mechanical stimulus
response to hormone
response to organic substance
tissue regeneration
response to amino acid
response to estrogen
negative regulation of membrane protein ectodomain proteolysis
response to folic acid
negative regulation of ERK1 and ERK2 cascade
cellular response to organic substance
positive regulation of TRAIL-activated apoptotic signaling pathway
negative regulation of metalloendopeptidase activity
negative regulation of peptidase activity
negative regulation of endopeptidase activity
response to cytokine
negative regulation of catalytic activity
negative regulation of proteolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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