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Product Details
PA5-20020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A peptide corresponding to 14 amino acids near the amino terminus of human TLR2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11151892
Product Specific Information
Despite its predicted molecular weight, TLR2 often migrates at ~100kDa in SDS-PAGE. A suggested positive control is A-20 cell lysate.
PA5-20020 can be used with blocking peptide PEP-0139.
Target Information
TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD282; TLR-2; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4
Gene Aliases: CD282; Ly105; TIL4; TLR2
UniProt ID: (Human) O60603, (Mouse) Q9QUN7
Entrez Gene ID: (Human) 7097, (Mouse) 24088
lipopolysaccharide binding
lipopolysaccharide receptor activity
receptor activity
transmembrane signaling receptor activity
protein binding
signaling pattern recognition receptor activity
Toll-like receptor binding
triacyl lipopeptide binding
peptidoglycan binding
protein heterodimerization activity
diacyl lipopeptide binding
lipoteichoic acid binding
lipopeptide binding
microglial cell activation
toll-like receptor signaling pathway
response to molecule of bacterial origin
response to molecule of fungal origin
cytokine secretion involved in immune response
immune system process
positive regulation of leukocyte migration
regulation of dendritic cell cytokine production
cell surface pattern recognition receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
leukotriene metabolic process
inflammatory response
immune response
signal transduction
I-kappaB phosphorylation
negative regulation of cell proliferation
response to bacterium
positive regulation of Wnt signaling pathway
central nervous system myelin formation
response to bacterial lipoprotein
response to peptidoglycan
interleukin-10 production
negative regulation of interleukin-12 production
negative regulation of interleukin-17 production
positive regulation of chemokine production
positive regulation of interferon-beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-18 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of toll-like receptor signaling pathway
toll-like receptor 2 signaling pathway
positive regulation of NF-kappaB import into nucleus
detection of triacyl bacterial lipopeptide
detection of diacyl bacterial lipopeptide
positive regulation of tumor necrosis factor biosynthetic process
chloramphenicol transport
negative regulation of growth of symbiont in host
innate immune response
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
nitric oxide metabolic process
positive regulation of oligodendrocyte differentiation
regulation of cytokine secretion
positive regulation of cytokine secretion
positive regulation of inflammatory response
defense response to Gram-positive bacterium
positive regulation of NF-kappaB transcription factor activity
positive regulation of nitric-oxide synthase biosynthetic process
induction by symbiont of defense-related host nitric oxide production
positive regulation of macrophage cytokine production
response to fatty acid
cellular response to bacterial lipopeptide
cellular response to lipoteichoic acid
cellular response to peptidoglycan
cellular response to diacyl bacterial lipopeptide
cellular response to triacyl bacterial lipopeptide
apoptotic process
positive regulation of gene expression
lipopolysaccharide-mediated signaling pathway
toll-like receptor TLR1:TLR2 signaling pathway
toll-like receptor TLR6:TLR2 signaling pathway
Performance Guarantee
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